MOLECULAR ANALYSIS OF COLLETOTRICHUM SPECIES ASSOCIATED TO OLIVE IN CALABRIA.

A new molecular approach based on the use of genus-specific primers targeting the internal transcribed spacer (ITS) regions of rDNA, was developed and used to study the diversity of Colletotrichum species associated with the olive canopy in the Gioia Tauro plain (Calabria, southern Italy). Representative symptomatic and symptomless samples of leaves, flowers and fruits were collected during 2012 and analyzed by extracting total DNA and amplifying the target region with the genus-specific primers. Amplicons were cloned and sequenced in order to use the ITS as a barcode gene. No Colletotrichum species were detected in the first sampling period (May 28, 2012), whereas around 15% of the analyze…

Phytophthora spp. in agrumeti unica malattia con più sintomi

A gene encoding for a polyketide synthase may affect the virulence of Diaporthe helianthi