0000000000883752

AUTHOR

Gerard Cagney

0000-0001-7189-9496

showing 1 related works from this author

Polycomb-like 2 Associates with PRC2 and Regulates Transcriptional Networks during Mouse Embryonic Stem Cell Self-Renewal and Differentiation

2010

SummaryPolycomb group (PcG) proteins are conserved epigenetic transcriptional repressors that control numerous developmental gene expression programs and have recently been implicated in modulating embryonic stem cell (ESC) fate. We identified the PcG protein PCL2 (polycomb-like 2) in a genome-wide screen for regulators of self-renewal and pluripotency and predicted that it would play an important role in mouse ESC-fate determination. Using multiple biochemical strategies, we provide evidence that PCL2 is a Polycomb Repressive Complex 2 (PRC2)-associated protein in mouse ESCs. Knockdown of Pcl2 in ESCs resulted in heightened self-renewal characteristics, defects in differentiation, and alte…

Pluripotent Stem CellsCellular differentiationGene regulatory networkDown-RegulationPolycomb-Group Proteinsmacromolecular substancesMethylationBiochemistryArticleCell LineHistonesSelf-RenewalMice03 medical and health sciences0302 clinical medicineEmbryonic Stem CellHistone methylationPolycomb-group proteinsGeneticsAnimalsGene Regulatory NetworksEpigeneticsInduced pluripotent stem cellEmbryonic Stem Cells030304 developmental biologyGenetics0303 health sciencesbiologyurogenital systemGene Expression ProfilingPolycomb Repressive Complex 2Cell DifferentiationCell BiologyCellular ReprogrammingSTEMCELLPRC2Embryonic stem cellRepressor ProteinsOncologyDifferentiation030220 oncology & carcinogenesisembryonic structuresbiology.proteinMolecular MedicineTranscriptional NetworkPRC2Genome-Wide Association StudyProtein BindingCell Stem Cell
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