In vitro effects of AGNPs exopolysaccharide from Klebsiella Oxytoca DSM29614 on breast cancer cells

Biological activities of AgNPs-EPS on breast cancer cells in vitro

CYTOTOXIC EFFECTS OF SILVER NANOPARTICLES (AgNPs) BIOSYNTHESIZED FROM KLEBSIELLA OXYTOCA DSM29614 AGAINST BREAST CANCER CELLS

Klebsiella oxytoca DSM 29614 (KO) is a strain that produces, under anaerobic conditions, bacterial exopolysaccharides (EPSs), made of four rhamnose (Rha), two glucuronic acids (GlcA) and one galactose (Gal) bound by α and β glycosidic bonds1,2, showing metal-binding properties3. In particular, KO in the presence of AgNO3 is able to synthesize silver nanoparticles (AgNPs) incorporated within the EPS (AgNPs-EPS). The AgNPs-EPS, may contain Ag+1 when KO growing in the presence of oxygen and Ag° under anaerobic conditions, giving a different biological activity4. In the present study were evaluated the cytotoxic effects of AgNPs-EPS, produced under aerobic and anaerobic conditions, on breast ca…