0000000000884826
AUTHOR
André Bouchot
showing 2 related works from this author
Automatic cell nuclei counting: a protocol to acquire images and to compare results between color and multispectral images
2013
International audience; High-throughput screening in histology and analysis need a necessary automatic cell or nucleus counting. Current methods and systems based on grayscale or color images give results with counting errors. We suggest to use multispectral imaging (with more than three bands) rather than color one for nucleus counting. A traditional acquisition chains uses a source of white light and a CCD camera in addition to the optical microscope. To pass to a multispectral acquisition, we use a Programmable Light Source (PLS) in the place of the white light source. This PLS is capable of generating different wavelengths in the visible spectrum. So, one color image and four multispect…
Flexibility of Thiel's embalmed cadavers: the explanation is probably in the muscles
2011
International audience; Introduction The flexibility of cadavers conserved using Thiel's embalming method remains unexplained. We aimed to perform microscopic comparison of muscle and tendon fibres from fresh cadavers (FC), formaldehyde-preserved cadavers (FPC) and cadavers conserved by Thiel's method (TC). Methods Muscle and tendon biopsies performed on FC, FPC and TC were conditioned and stained by Masson's trichrome, Sirius red and Ramon y Cajal, then studied under optical microscope. Alignment and integrity of the muscle and tendon fibres were studied. Results We observed a modification of the muscle fibres in all specimens from TC, regardless of the type of staining used. The muscle fi…