Development and validation of a procedure for estimating the hydrophobicity of structurally unrelated compounds by micellar liquid chromatography

Reversed-phase liquid chromatography has been used most often to estimate values of log P, but despite years of study, there is no universally accepted method of performing these estimations. The main problem has to do with the fact that the hydrophobic parameter, log k w , depends on the hydrogen bond acceptor-donor character of the compounds. The use of micellar mobile phases to perform these estimations is evaluated here, and the influence of the nature of the surfactant (anionic, cationic, and nonionic) on the log k-log P relationships is studied. The use of a nonionic surfactant, such as Brij35, to prepare the mobile phases provided adequate results regardeless of the hydrogen bond acc…