IMMUNOHISTOCHEMICAL DISTRIBUTION OF CONSTITUTIVE NOS ISOFORMS IN PROSTATE, SEMINAL VESICLES AND BULBOURETHRAL GLANDS IN SOME MAMMALS MALE GENITAL TRACT

Caspase 9 and Caspase 3 Immunohistochemical Pattern in Skeletal and Cardiac Muscles at Different Times after Death: An Experimental Study on PMI Estimation

(1) Background: The estimation of the post mortem interval (PMI) is a challenge for forensic pathologists because data emerging from methods commonly applied are not always conclusive, since several conditions exist that may affect the reliability of these parameters. Thus, new approaches have been proposed to overcome such a limit. In recent years, several studies have been performed on proteins analyzing their expression/degradation patterns in relation to the progressing of the post mortem interval. (2) Methods: The immunoreactivity patterns of two apoptosis mediators—Caspase 9 and Caspase 3—have been tested in order to evaluate their potential role as markers of the post mortem interval…

Immunohistochemical and molecular expression of CB1 receptors in human colonic segment. Preliminary results

Recent studies document that CB1 cannabinoid receptors, considered since its first identification as “brain specific”, could be expressed by peripheral tissues targets (adipose organs, enteric nervous system, striated muscle, epatocytes). This receptors were found in the GI tract of different species, including mice, rats, guinea pigs, pigs and humans. Endocannabinoids play a role in the pathophysiology of the gastrointestinal tract. They play a role in emesis, acid-related disorders, motility-related disorders, irritable bowel syndrome and diarrhea, and in inflammatory bowel disease. Endocannabinoid system might be involved in cancer differentiation, growth and cell migration. Distribution…

In Naive mice in vivo localization of WI specific CD8+CD28- induced cells

Immunohistochemical expression of Cytokeratins in periapical lesions

Objective. This study seeks to clarify the histological origin of apical lesions, through the expression of certain cytokeratins.Study design. In 3 years 30 patients were selected. After clinical and radiological exams were chosen patients who had severe apical lesions and tooth decay with exposure of the pulp chamber left untreated for very long periods (more the 12 months). Serial slides were prepared both for immunohistochemistry and Hematoxylin- Eosin. Material and methods. Twenty specimens coming from the 30 patients were used for our purpose. All periapical lesions were surgically extracted and fixed in Bouin mixture, and embedded in paraffin. Samples were processed immunohistochemica…

Effect of chronic treatment with CB1 receptor antagonist (Rimonabant) on B-cells apoptosis in the pancreas of obese Zucker rats. An immunohistochemical study

Preliminary morpho-structural studies during endodontic treatment with Ni-Ti 55-45 instruments

Risk of villous histology or high grade dysplasia in patients with diminutive polyps: influence of number of polyps

INTRODUCTION/OBJECTIVES: Current recommendations on post-polypectomy surveillance recommend shorter intervals in case of 3 or more polyps, irrespectively of histological findings (see reference below). These recommendations doesn't differentiate between diminutive (1-5 mm) polyps and polyps of larger size (>5 mm). AIMS & METHODS: Aim of the present study was to assess, in a group of patients with "isolated" diminutive polyps, i.e. without associated polyps of larger size, if the presence of 3 or more diminutive polyps is associated with higher number of polyps with villous histology (VH) or high grade dysplasia (HGD). Retrospective examination of endoscopical records and of histopathologica…

Immunohistochemical expression and apoptotic role of Caspase9 in aortic aneurysms

Immunhistochemical expression of some neuropeptides and CB1 cannabinoid receptor in rat lung. Note II: Trachea and Bronchi

IMMUNOHISTOCHEMICAL DISTRIBUTION OF ORPHANIN FQ IN THE RAT GASTROINTESTINAL APPARATUS AND PANCREAS

Modulation of MMP-2 and MMP-9 in Churg-Strauss syndrome respiratory mucosa: potential monitoring parameters.

Churg-Strauss (CSS) syndrome is rare and of unknown etiology. It is associated with vasculitis, blood eosinophilia and granulomatosis, and affects multiple organs and systems at various stages of the disease. Specific diagnostic and monitoring tests are not yet available. This study aims to assess the changes in MMP-2 and MMP-9 along with the histopathological alterations in two cases of CSS, as possible potential diagnostic and monitoring criteria. Two adult male patients were diagnosed with CSS in the otorhinolaryngology clinic in the University of Palermo, based on multiple clinical and histopathologic criteria. Biopsies of respiratory mucosa were taken after the consent of the patients…

Natural autofluorescence in archeological bone

The human bone present a natural autofluorescence which is tipically induced by natural antibiotiscs absorbed by collagen and in part is due to phenomena not weel know and referred to an enigmatic endogenous fluophore. Is iintristing for us to see the autofluorescence in the various age of the observed samples.

Expression of Nitric Oxide Synthase in Inflamed Gingival Tissue: Immunoistochemical and Image Analysis

Immunohistochemical expression of iNOS and eNOS in the venous ulcers related to chronic venous insufficiency (CVI).

The Essential of Bone Histology for Forensic Applications

This chapter illustrates characteristics of bone, as specialized connective tissue, taking into account fundamentals of histology for forensic purposes (Cells of Bone, Concentric or Haversian system, Systems of internal and external circumferential lamellae, Bone Remodeling).

Immunohistochemical expression of apoptotic factors, cytokeratins, and metalloproteinase-9 in periapical and epithelialized gingival lesions

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Comparative analysis of Hsp10 and Hsp90 in large bowel healthy mucosa and adenocarcinomas

Heat shock proteins (Hsps) are an important class of molecules with various functions. Their classic role is to assist other proteins in folding and re-folding and, when proteins are defective or irreversibly misfolded, to drive their degradation. For this reason, some Hsps are also named molecular chaperones. During evolution, this class of proteins has also acquired extrachaperoning roles such as participation in immune system regulation, cell differentiation, programmed cell death and carcinogenesis. Hsp10 is a partner of Hsp60 in the Hsp60/10 folding machine, but numerous scientific studies have shown that Hsp10 may also play other roles. In fact, Hsp10 seems to have an immunomodulatory…

Bovine seminal vesicles ANP, Oxytocin, immunohistochemical expression.

Il controllo nitrossidergico e peptidergico della secrezione salivare nella parotide di alcuni mammiferi

Clinical Significance Of Isolated Diminutive Colonic Polyps

INTRODUCTION/OBJECTIVES: Several reports have assessed clinical significance of diminutive (1-5 mm) polyps; here we report on the prevalence of advanced histology in patient with "isolated" diminutive polyps, i.e. without associated polyps of larger size. AIMS & METHODS: Retrospective examination of endoscopy records and of histopathology records of patients with polyps of any size. Advanced histology was defined as the presence of high grade dysplasia (HGD) or in situ carcinoma. Polyps were categorized into three groups: diminutive (1-5 mm); small (6-10 mm) and large (11 mm or more). Diminutive Small Large Advanced Carcinoma Number 315 89 67 84 Male (%) 205 (62) 57 (64) 46 (68) 52 (62) Mea…

NO and Chronic Venous Insufficiency (CVI): Immunohistochemical Evaluation of iNOS and eNOS Isofroms in the Venous Ulcers. Clinical and Therapeutical Possible Implications

Wharton’s Jelly Mesenchymal Stromal Cells from Human Umbilical Cord: a Close-up on Immunomodulatory Molecules Featured In Situ and In Vitro

Therapeutic options for end-stage organ failure are often limited to whole organ transplantation. The tolerance or rejection of the transplanted organ is driven by both early non-specific innate and specific adaptive responses. The use of mesenchymal stromal cells (MSCs) is considered a promising tool in regenerative medicine. Human umbilical cord (HUC) is an easily available source of MSCs, without relevant ethical issues. Moreover, Wharton's jelly-derived MSCs (WJ-MSCs), showed consistent immunomodulatory features that may be useful to promote immune tolerance in the host after transplantation. Few data are available on the phenotype of WJ-MSCs in situ. We investigated the expression of i…

A possible biomarker for methadone related deaths

Abstract Methadone (MTH) concentrations in those dying of MTH toxicity totally overlap concentrations where the presence of MTH is only an incidental finding, making it very difficult to make distinctions in actual cases. A biomarker, be it anatomical or biochemical for MTH toxicity is badly needed, particularly if that markers were known to disrupt effective ventilation. Because the brainstem houses the regulatory centers for cardiorespiratory-control enters, it would seem to be the most likely anatomical site to seek abnormalities in cardiorespiratory control. Objective To locate and describe the cells of nucleus of the solitary tract (TS)(NTS) in human brainstem and determine if neuronal…

Immunohistochemical Expression of NO-synthase Constitutive Isoform (cNOS)in Mammals Normal Prostate and in Human Prostatic Obstructive Hyperplasia

The aim of present study is to investigate the immunohistochemical identification of cNOS-containing prostatic structures in various species and in hyperplastic human prostate. We used specimens of rat, guinea pig and hamster. Human prostatic specimens were removed from patients suffering of prostatic hyperplasia. The study was processed by ABC method. Immunohistochemical staining with anti nNOS antibody revealed the existence of strong reactivity in numerous ganglionic cell bodies and varicose nerve fibers in the fibromuscular stroma, in smooth cells around acini and in blood vessels endothelium. Surprisingly nNOS immunoreactivity appears in the glandular epithelium. In the hyperplastic pr…

Immunohistochemical expression of CB1 cannabinoid receptor in the laryngeal-tracheal tract in humans suffering from laryngeal neoplasia

Caspase 9 and Caspase 3 Immunohistochemical Reactivity Pattern in Skeletal and Cardiac Muscle at Different Times After Death: A New Tool for Postmortem Interval (PMI) Determination?

Learning Overview: After attending this presentation, attendees will understand the importance of the identification of new markers that could be used as possible PMI indicators, especially in contexts in which the reliance on the classic thanatochronological triad—livor, rigor, and algor mortis—is not conclusive. Impact on the Forensic Science Community: This presentation will impact the forensic science community by highlighting the relationship between the activation pattern of caspases 9 and 3 on skeletal and cardiac muscle samples and PMI. The determination of the amount of time elapsed from one’s death to the recovery of the body—the so-called PMI—has always relied on the evaluation o…

Immunohistochemical and Biomolecular Identification of Orphanin FQ, eNOS, Atrial natriuretic Factor and Oxytocin in Rat Seminal Vesicles

In previous studies performed on rodents, we detected the presence of adreno-cholinergic and peptidergic innervation in seminal vesicles and other organs of the male genital system, such as prostate and ductus deferens, in which we also investigated the expression of NOS and NADPH-diaphorase. During the present project we focused our attention on the expression of some peptides involved in local control of smooth muscle relaxation, contractility, vasodilatation and control of blood flow in rat seminal vesicles. We investigated, through immunohistochemistry and RT-PCR, the presence of four peptides: orphanin, eNOS, ANF and oxytocin. Immunohistochemistry was used to detect the presence of the…

Immunhistochemical studies on leptin, ghrelin, orexin and orphanin fq expression in the stomach of obese (fa/fa)zucker rats

Expression of Nitric Oxide Synthase in inflamed gingival tissue: immunohistochemical and image analysis

We have used 12 Epulis taken during surgical operation and as control, some normal gingival specimen. The aim was to clarify the relationship between the degree of inflammation and NO production in several cases of Epulis, exhibiting different degree of inflammatory process and verifying the presence of cells involved in NO production. From the immuhistochemical data in our possession we can conclude that NO is one of the factor which module the pathogenecity degree of Epulis.

Immunohistochemical expression of orphanin FQ, atrial natriuretic peptide and oxytocin in normal human seminal vesicles

Immunohistochemical expression of iNOS and metalloproteinases MMP2 and MMP9 in abdominal inflammatory aortic Aneurysms

COVID-19 Vaccine-Related Thrombosis: A Systematic Review and Exploratory Analysis

IntroductionThe World Health Organization declared the coronavirus disease 2019 (COVID-19) pandemic on March 11, 2020. Two vaccine types were developed using two different technologies: viral vectors and mRNA. Thrombosis is one of the most severe and atypical adverse effects of vaccines. This study aimed to analyze published cases of thrombosis after COVID-19 vaccinations to identify patients’ features, potential pathophysiological mechanisms, timing of appearance of the adverse events, and other critical issues.Materials and MethodsWe performed a systematic electronic search of scientific articles regarding COVID-19 vaccine-related thrombosis and its complications on the PubMed (MEDLINE) d…

Immunohistochemical expression of CB1 cannabinoids receptor in rat lung

The role of new neurocrine and neuroendocrine messengers on the regulation of the function of major salivary glands in some mammals. An immunohistochemical study

Preliminary observations on the immunohistochemical distribution of nNOS in the development of human foetus eye

Immunohistochemical expression of Orphanin FQ in the rat male genital tract

Immunohistochemical distribution of NOS Isoform in the Embryofoetal structures of Human encephalon

EFFETS D'UN DESENSIBILISANT DENTINAL A BASE D' OSSALATE FERRIQUE: ANALISY AU SEM BULLETIN DU GIRSO

CB1 receptor expression in pancreatic islet of the obese and diabetes Zucher rats

The chronical gastritis Helicobacter pylori correlated: immunohistochemical behaviour of nitric oxide synthase isoforms in rat's stomach

Recent non extensive studies indicate that Nitric Oxide (NO), a novel intercellular messenger, may play a role in pathogenesis of gastric lesions by Helicobacter pylori (Hp). Aim of this study is to evaluate the immunohistochemical distribution and modification of NO synthases (cNOS - iNOS) in experimentally induced gastritis by Hp in rats.

Immunohistochemical evidence for the presence of CB1 cannabinoid receptor in rat salivery glands

Emerging Immnunohistochemical evidence for Direct Peripheral Control of Endocannabinoids on the Gastrointestinal Tract and Pancreas of Obese (fa/fa)and Lean Zucker Rats (Pathophysiological Implications)

This research has the objective to investigate immunohistochemical expression of CB1 receptor and its probable changes in Gastroenteropancreatic system (GEP) of obese and lean Zucker rats and understand the endocannabinoid pathophysiological implications in the obesity. Male obese (fa/fa) and lean Zucker rats 6 weeks old were obtained from Harlan Italy Srl; the rats were sacrificed at 8, 12 and 16 weeks old. Normal rats also were sacrificed. Specimens of stomach, jejunum-ileum and pancreas were fixed in Bouin’s mixture and embedded in paraffin; obtained sections were processed with anti-CB1 (Biosource Europe SA) by Streptavidin-Biotin-Complex Method. The findings show that CB1 receptor is e…

Dental pulp in mature human teeth: morphological alterations and metalloproteinases-2 and -9, Annexin-5, BCL-2, and iNOS modulation.

Tooth replantation, as a treatment concept, has been subject to controversies regarding the mechanism as well as the various parameters underlying this process. This work aimed to study time-related changes in the pulp of replanted mature human premolars through the changes in the levels of certain factors involved in the underlying mechanisms of pulpal tissue healing after replantation. Eleven experimental mature teeth were extracted, immediately replanted in the original socket and left without any other intervention for 1, 2, 3 and 12 weeks before re-extraction. Three premolars served as control. All specimens were subject to histological analysis and the levels of MMP-2, MMP-9, Annexin …

Immunohistochemical expression of Orexin A and Orexin Type 2 receptors in the rat gastrointestinal apparatus and endocrine pancreas

Orthodontic Stress BCL-2 Nodulation and Human Odontoblast's Survival

Immunohistochemical expression and distribution of orexin, orphanin and leptin in the major salivary glands of some mammals

Abstract: The aim of the study was to assess the involvement of apoptotic factors, cytokeratins and metalloproteinase- 9 in the histogenesis of both Epithelialized Gingival Lesions (EGL) and Periapical Lesions (PAL). 55 consecutive patients, 30 with PAL and 25 with EGL, were selected for the study after clinical and radiological examinations. The PAL patients had severe periapical lesions and tooth decay with exposure of the pulp chamber. All PAL and EGL biopsies were surgically extracted, fixed in 10% buffered formalin, and processed for routine light microscopy. Ten biopsies of each category were processed for immunohistochemistry (IHC). Serial paraffin sections were stained by IHC with a…

Expression of Remodelling Enzymes MMPs in Hernia Formation

An immunohistochemical study on the cb1 receptor expression in rat salivary glands

Orphanin FQ and motility in seminal vesicles