0000000000905399

AUTHOR

Shinya Ohata

showing 3 related works from this author

Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

2015

Directional beating of ependymal (E) cells9 cilia in the walls of the ventricles in the brain is essential for proper CSF flow. E cells display two forms of planar cell polarity (PCP): rotational polarity of individual cilium and translational polarity (asymmetric positioning of cilia in the apical area). The orientation of individual E cells varies according to their location in the ventricular wall (location-specific PCP). It has been hypothesized that hydrodynamic forces on the apical surface of radial glia cells (RGCs), the embryonic precursors of E cells, could guide location-specific PCP in the ventricular epithelium. However, the detection mechanisms for these hydrodynamic forces hav…

Ependymal Cellurogenital systemGeneral NeuroscienceCiliumciliaplanar cell polarityBiologyurologic and male genital diseasesEmbryonic stem cellNeural stem cellEpitheliumfemale genital diseases and pregnancy complicationsCell biologyneural stem cellmedicine.anatomical_structureCell polaritymedicinepolycystinEpendymaepitheliumEpendymoglial Cellsependymal cell
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Loss of Dishevelleds disrupts planar polarity in ependymal motile cilia and results in hydrocephalus.

2014

Defects in ependymal (E) cells, which line the ventricle and generate cerebrospinal fluid flow through ciliary beating, can cause hydrocephalus. Dishevelled genes (Dvls) are essential for Wnt signaling, and Dvl2 has been shown to localize to the rootlet of motile cilia. Using the hGFAP-Cre;Dvl1(-/-);2(flox/flox);3(+/-) mouse, we show that compound genetic ablation of Dvls causes hydrocephalus. In hGFAP-Cre;Dvl1(-/-);2(flox/flox);3(+/-) mutants, E cells differentiated normally, but the intracellular and intercellular rotational alignments of ependymal motile cilia were disrupted. As a consequence, the fluid flow generated by the hGFAP-Cre;Dvl1(-/-);2(flox/flox);3(+/-) E cells was significant…

Neuroscience(all)Dishevelled ProteinsMice TransgenicBiologyTransgenicArticleMiceEpendymaCell polarityFLOXGeneticsmedicinePsychologyAnimalsCiliaAdaptor Proteins Signal Transducingchemistry.chemical_classificationNeurology & NeurosurgeryGeneral NeuroscienceCiliumSignal TransducingNeurosciencesWnt signaling pathwayAdaptor ProteinsCell PolarityPhosphoproteinsDishevelledCell biologymedicine.anatomical_structurechemistryMotile ciliumCognitive SciencesEpendymaIntracellularHydrocephalusSignal TransductionNeuron
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Ependymoma associated protein Zfta is expressed in immature ependymal cells but is not essential for ependymal development in mice

2022

AbstractThe fusion protein of uncharacterised zinc finger translocation associated (ZFTA) and effector transcription factor of tumorigenic NF-κB signalling, RELA (ZFTA-RELA), is expressed in more than two-thirds of supratentorial ependymoma (ST-EPN-RELA), but ZFTA’s expression profile and functional analysis in multiciliated ependymal (E1) cells have not been examined. Here, we showed the mRNA expression of mouse Zfta peaks on embryonic day (E) 17.5 in the wholemount of the lateral walls of the lateral ventricle. Zfta was expressed in the nuclei of FoxJ1-positive immature E1 (pre-E1) cells in E18.5 mouse embryonic brain. Interestingly, the transcription factors promoting ciliogenesis (cilia…

CNS cancerMultidisciplinaryEpendymomaScienceQRMedicineDevelopment of the nervous systemArticle
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