MICROFLUIDIC PROCEDURE FOR FABRICATION OF HYALURONIC ACID BASED BIOMATERIALS

UPTAKE OF SILICA COVERED QUANTUM DOTS ON LIVING CELLS: LONG TERM VITALITY AND MORPHOLOGY STUDY ON HYALURONIC ACID BIOMATERIALS

In this work we prepared colloidal CdSe-CdS core-shell nanocrystal emitting @625 nm and, by a micro-emulsion procedure of water in oil inverse micelle, we encapsulated a single dot inside each silica sphere. In this way the nanoparticles became biocompatible, are simply to prepare and could be used also in a water based environment. Moreover, the silica shield is transparent to the wavelengths of interest.

MULTIFUNCTIONAL HYALURONIC ACID DERIVATIVE FOR EASY SCAFFOLD PROCESSING AND BIOLOGICAL FUNCTIONALIZATION

POLISACCARIDI FUNZIONALIZZATI CON PORZIONI ALCHILICHE: SINTESI, CARATTERIZZAZIONE REOLOGICA ED APPLICAZIONI BIOMEDICHE

Fibrillar polymeric patches suitable for wound healing

Multilayered scaffolds can be designed to improve soft material applicability for regenerative medicine purposes. Excellent results can be obtained by combining the main characteristics of the biopolymers and giving a specific function to each layer. A microfluidic-assisted electrospinning process is an elegant arrangement between two fiber fabrication techniques that defines finely every portion of an electrospun scaffold. Unlike classic electrospinning technique, multiple flows can be carefully monitored and separated in real time using microfluidic sensors. This method allows users to establish anisotropic properties to scaffolds, in other words it is possible to modulate gradients by ch…

HYALURONIC ACID/-CYCLODEXTRINS HYDROGEL FOR CORNEAL RELEASE OF EPITHELIAL CELLS AND DEXAMETHASONE

INJECTABLE HYDROGEL BASED ON A NOVEL AMPHIPHILIC HYALURONIC ACID DERIVATIVE FOR CONTROLLED DRUG RELEASE

SPRAY DRIED MICROPARTICLES OF A NEW HYALURONIC ACID DERIVATIVE FOR POTENTIAL BIO-INSTRUCTION OF STAMINAL CELLS

HYALURONIC ACID-BETA CYCLODEXTRINS HYDOGEL FOR THE TREATMENT OF SEVERE CORNEAL DISEASES

PRODUCTION VIA MICROFLUIDICS OF FIBRILLAR AND NANOMETRIC BIOMATERIALS FOR REGENERATIVE MEDICINE AND DRUG DELIVERY POURPOSES

Microfluidic technique offers mild operating conditions suitable for fabrication of fibers and cell encapsulation and can be applied for controlled production of nanometric biomaterials1 . Ionotropic polysaccharides are routinely employed for microfluidics. To be processed, nonionotropic polysaccharides, such as Hyaluronic Acid (HA), should be chemically crosslinked during fabrication2 . In order to avoid chemical crosslinking, we designed an ionic strenght sensible HA derivative suitable for microfluidics2 . Here we describe the use of such HA derivative for fabrication of fibrillar and nanometric biomaterials for regenerative medicine and drug delivery applications. For muscle tissue rege…

Biocompatibility and biodegradability of electrospun phea-pla scaffolds: Our preliminary experience in a murine animal model

We obtained a nano-fibrillar scaffold starting from a polymeric solution which, through electrospinning, gave a biodegradable material with optimal mechanical features and the capacity to allow cell adhesion. In this paper we report the in-vivo application on a murine animal model of two electrospun biodegradable materials, specifically designed to create tubular structures. In one case PHEA-PLA was co-spun with silk fibroin (Fibro-PHEAPLA) by a parallel electrospinning process to obtain a scaffold with two different polymeric fibers. In the other case, PHEA-PLA was mixed with polycaprolactone (PCLPHEA-PLA) to obtain a hybrid fibers scaffold. The in-vitro assay showed fibroblast colonizatio…

New polysaccharide-polyaminoacid hydrogels: a suitable strategy for colon-specific drug delivery

The aim of this study was to prepare and characterize novel hydrogels with polysaccharide–polyaminoacid structure, able to undergo an enzymatic hydrolysis in the colon and potentially useful for treating inflammatory bowel diseases (IBD). Starting materials were methacrylated dextran (DEX-MA) and methacrylated α,β-poly(N-2-hydroxyethyl)-DL-aspartamide (PHM). These polymers were photocrosslinked by exposure of their aqueous solutions at 313 nm without photoinitiators. Different samples, shaped as microparticles, were obtained as a function of polymer concentration and irradiation time. FT-IR analysis confirmed the occurrence of a co-crosslinking between DEX-MA and PHM in all experimental con…

SPONGES OF NEW HYALURONIC ACID DERIVATIVES FOR BIOMEDICAL APPLICATIONS