NEW BIODEGRADABLE HYDROGELS DESIGNED FOR COLONIC DRUG DELIVERY: IN VITRO RELEASE OF THE PEPTIDES GLUTATHIONE AND OXYTOCIN

MICROFLUIDIC PROCEDURE FOR FABRICATION OF HYALURONIC ACID BASED BIOMATERIALS

UPTAKE OF SILICA COVERED QUANTUM DOTS ON LIVING CELLS: LONG TERM VITALITY AND MORPHOLOGY STUDY ON HYALURONIC ACID BIOMATERIALS

In this work we prepared colloidal CdSe-CdS core-shell nanocrystal emitting @625 nm and, by a micro-emulsion procedure of water in oil inverse micelle, we encapsulated a single dot inside each silica sphere. In this way the nanoparticles became biocompatible, are simply to prepare and could be used also in a water based environment. Moreover, the silica shield is transparent to the wavelengths of interest.

Analisi quantitativa dell'effetto termoelastico in materiali compositi CFRP

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge. New…

MULTIFUNCTIONAL HYALURONIC ACID DERIVATIVE FOR EASY SCAFFOLD PROCESSING AND BIOLOGICAL FUNCTIONALIZATION

POLISACCARIDI FUNZIONALIZZATI CON PORZIONI ALCHILICHE: SINTESI, CARATTERIZZAZIONE REOLOGICA ED APPLICAZIONI BIOMEDICHE

Fibrillar polymeric patches suitable for wound healing

Multilayered scaffolds can be designed to improve soft material applicability for regenerative medicine purposes. Excellent results can be obtained by combining the main characteristics of the biopolymers and giving a specific function to each layer. A microfluidic-assisted electrospinning process is an elegant arrangement between two fiber fabrication techniques that defines finely every portion of an electrospun scaffold. Unlike classic electrospinning technique, multiple flows can be carefully monitored and separated in real time using microfluidic sensors. This method allows users to establish anisotropic properties to scaffolds, in other words it is possible to modulate gradients by ch…

HYALURONIC ACID/-CYCLODEXTRINS HYDROGEL FOR CORNEAL RELEASE OF EPITHELIAL CELLS AND DEXAMETHASONE

A quantitative investigation on the thermoelastic effect of CFRP laminates

The thermoelastic effect on CFRP laminates with various lay-ups is investigated. A thick low crimp unidirectional fabric reinforcement is adopted. The measured thermoelastic signal is compared with predictions from two analytical models based on the meso-mechanical bulk properties of the lamina and on assuming a strain witness behaviour of the surface resin rich layer.

INJECTABLE HYDROGEL BASED ON A NOVEL AMPHIPHILIC HYALURONIC ACID DERIVATIVE FOR CONTROLLED DRUG RELEASE

COMPLEXES OF IRON WITH INULIN DERIVATIVES: A NEW STRATEGY IN THE TREATMENT OF IRON DEFICIENCY ANAEMIA

SPRAY DRIED MICROPARTICLES OF A NEW HYALURONIC ACID DERIVATIVE FOR POTENTIAL BIO-INSTRUCTION OF STAMINAL CELLS

HYALURONIC ACID-BETA CYCLODEXTRINS HYDOGEL FOR THE TREATMENT OF SEVERE CORNEAL DISEASES

PRODUCTION VIA MICROFLUIDICS OF FIBRILLAR AND NANOMETRIC BIOMATERIALS FOR REGENERATIVE MEDICINE AND DRUG DELIVERY POURPOSES

Microfluidic technique offers mild operating conditions suitable for fabrication of fibers and cell encapsulation and can be applied for controlled production of nanometric biomaterials1 . Ionotropic polysaccharides are routinely employed for microfluidics. To be processed, nonionotropic polysaccharides, such as Hyaluronic Acid (HA), should be chemically crosslinked during fabrication2 . In order to avoid chemical crosslinking, we designed an ionic strenght sensible HA derivative suitable for microfluidics2 . Here we describe the use of such HA derivative for fabrication of fibrillar and nanometric biomaterials for regenerative medicine and drug delivery applications. For muscle tissue rege…

Biocompatibility and biodegradability of electrospun phea-pla scaffolds: Our preliminary experience in a murine animal model

We obtained a nano-fibrillar scaffold starting from a polymeric solution which, through electrospinning, gave a biodegradable material with optimal mechanical features and the capacity to allow cell adhesion. In this paper we report the in-vivo application on a murine animal model of two electrospun biodegradable materials, specifically designed to create tubular structures. In one case PHEA-PLA was co-spun with silk fibroin (Fibro-PHEAPLA) by a parallel electrospinning process to obtain a scaffold with two different polymeric fibers. In the other case, PHEA-PLA was mixed with polycaprolactone (PCLPHEA-PLA) to obtain a hybrid fibers scaffold. The in-vitro assay showed fibroblast colonizatio…

Experimental evaluation of through-the-thickness stress distribution in transverse crack tension test samples

Transverse Crack Tension (TCT) samples represent an alternative to End Notched Flexure configurations for the characterization of pure mode II fracture toughness of Fibre Reinforced Polymer composites. TCT is supposed to reproduce a pure Mode II failure mechanism while avoiding the frictional energy dissipation between the flanks of a starter crack. The TCT geometry is that of a unidirectional tensile sample where a number of embedded plies, centered on the middle plane, are cut in the transverse fibres direction. The tensile loading should then activate four symmetric fronts of mode II delamination from the transverse cut tips. The present work describes the implementation of two full fiel…

Mode I fracture toughness behaviour of epoxy resincarbon fiber composites cured by ionising radiation

In this work Mode I fracture toughness behavior of Electron Beam cured unidirectional CFRP laminates is investigated by means of standardized Double Cantilever Beam (DCB) tests. A matrix system, comprising a DGEBA epoxy monomer and a typical initiator of cationic polymerization, was used and panel samples assembled by hand lay-up. Curing was achieved by 20 to 40 minutes irradiation on a pulsed 10 MeV Electron Beam accelerator. One batch of resin was also mixed with 10 phr of a PES thermoplastic monomer in order to enhance the matrix toughness. The influence of fibre-matrix adhesion, matrix toughening and matrix crosslinking density on the mode I delamination behavior have been investigated …

New polysaccharide-polyaminoacid hydrogels: a suitable strategy for colon-specific drug delivery

The aim of this study was to prepare and characterize novel hydrogels with polysaccharide–polyaminoacid structure, able to undergo an enzymatic hydrolysis in the colon and potentially useful for treating inflammatory bowel diseases (IBD). Starting materials were methacrylated dextran (DEX-MA) and methacrylated α,β-poly(N-2-hydroxyethyl)-DL-aspartamide (PHM). These polymers were photocrosslinked by exposure of their aqueous solutions at 313 nm without photoinitiators. Different samples, shaped as microparticles, were obtained as a function of polymer concentration and irradiation time. FT-IR analysis confirmed the occurrence of a co-crosslinking between DEX-MA and PHM in all experimental con…

A NEW WATER-SOLUBLE FLUORINATED POLYMER BASED ON A POLYASPARTAMIDE AND OXADIAZOLE UNITS AS A POTENTIAL ARTIFICIAL OXYGEN (O2) CARRIER

idrogelo a base di acido ialuronico e suo uso in ortopedia

AMPHYPHILIC DERIVATIVES BETWEEN HYALURONIC ACID AND POLYLACTIC ACID

A NEW BIODEGRADABLE HYDROGEL FOR INTESTINAL SPECIFIC DRUG DELIVERY

The effect of the through-the-thickness compressive stress on mode II interlaminar fracture toughness

The effect of through-the-thickness compressive stress on the mode II interlaminar fracture toughness is investigated experimentally and replicated numerically. The modified Transverse Crack Tensile specimen recently proposed by the authors is used, together with an experimental device designed to apply a constant transverse compressive stress on the surface of the specimen. Experiments are conducted using IM7/8552 specimens for different compressive stresses, ranging from 0 to 100 MPa, covering all the practical applications commonly encountered in the aeronautical industry (e.g., tightened filled holes or bolted joints). It is shown that the mode II interlaminar fracture toughness increas…

SPONGES OF NEW HYALURONIC ACID DERIVATIVES FOR BIOMEDICAL APPLICATIONS