Author: Maurizio Manno

0000000000921997

AUTHOR

Maurizio Manno

showing 1 related works from this author

Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection

2005

Objective To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure. Design Comparison of the rate of DNA fragmentation in human oocytes at different stages of maturation soon after pick-up (control) and in unfertilized oocytes after ICSI treatment. Setting In vitro fertilization (IVF) laboratory with extensive ICSI experience. Patient(s) Sixty-three patients undergoing assisted fertilization by ICSI. Intervention(s) Terminal deoxynucleotidyl transferase-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay and anticaspase-3 cleaved…

virgin oocytesmedicine.medical_treatmentintracytoplasmic sperm injectionApoptosisDNA FragmentationBiologyICSIIntracytoplasmic sperm injectionAndrologyHuman fertilizationhuman oocyteConfidence IntervalsmedicineHumansSperm Injections Intracytoplasmicreproductive and urinary physiologyFertilisationApoptosis; fertilization failure; intracytoplasmic sperm injection; ICSI; human oocytes; virgin oocytesChi-Square DistributionIn vitro fertilisationTUNEL assayurogenital systemfertilization failureApoptosiObstetrics and GynecologyOocyteSpermmedicine.anatomical_structureReproductive MedicineOocytesDNA fragmentationFemaleFertility and Sterility

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