0000000000922837

AUTHOR

Pietro Di Fazio

“Acetilazione di p53 e degli istoni nell’apoptosi indotta dal SAHA in cellule di epatoma umano HepG2”

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THE NOVEL HDAC-INHIBITOR PANOBINOSTAT INHIBITS GROWTH OF HUMAN HEPATOCELLULAR CARCINOMA XENOGRAFTS IN NUDE MICE.

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The mechanism by which histone deacetylase inhibitors sensitize hepatoma and colon cancer cells to TRAIL-induced apoptosis

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The sensitization of HepG2 and HT29 cells to TRAIL-induced apoptosis by histone deacetylase inhibitors is mediated by down-regulation of AKT and NF-kB.

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Histone deacetylase inhibitors: Epigenetic drugs acting by pleiotropic apoptotic mechanism in tumor cells and highly potent in combination with other antitumor agents.

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THE ROLE OF THE EXTRINSIC PATHWAY OF APOPTOSIS IN THE EFFECT INDUCED BY SAHA IN HUMAN HEPATOMA CELLS.

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Tumor models: in vivo applications

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Flow Cytometry Study of Apoptosis

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The histone deacetylase inhibitor suberoylanilide hydroxamic acid sensitizes human hepatocellular carcinoma cells to TRAIL-induced apoptosis by TRAIL-DISC activation

Abstract This paper shows that the histone deacetylase inhibitor SAHA sensitised at sub-toxic doses human hepatocellular carcinoma cells (HepG2, Hep3B and SK-Hep1) to TRAIL-induced apoptosis, while it was ineffective in primary human hepatocytes (PHHs). In particular in HCC cells SAHA increased the expression of death receptor 5 (DR5) and caused a decrement of c-Flip. These two modifications provoked in the presence of TRAIL the rapid production of TRAIL-DISC and the activation of caspase-8. Consequently SAHA/TRAIL combination induced many apoptotic events, such as a cleavage of Bid into tBid, dissipation of mitochondrial membrane potential, activation of caspase-3 with the consequent cleav…

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“Effects of histone deacetylase inhibitors in human hepatoma cells and synergistic apoptotic interaction with the proteasome inhibitor Bortezomib”

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THE APOPTOTIC EFFECT EXERTED IN HUMAN HEPATOMA CELLS BY THE INHIBITOR OF HISTONE DEACETYLASE SAHA EITHER ALONE OR IN COMBINATION WITH BORTEZOMIB.

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“Human epithelial corneal cells with a high self-renewing potential: an in vitro model for ocular surface reconstruction”.

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HISTONE DEACETYLASE INHIBITORS SENSITIZE HEPATOMA CELLS TO TRAIL_INDUCED APOPTOSIS

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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1

Contains fulltext : 232759.pdf (Publisher’s version ) (Closed access) In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to…

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Autophagy

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide…

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“In cellule di osteosarcoma umano MG-63, il trattamento con 3-AB, inibitore della Parp, inibisce il segnale mediato da ILK/GSK-3/Beta-catenina/LEF-1”

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CAMPTOTHECIN INDUCES S-PHASE ARREST AND APOPTOSIS IN MG63 OSTEOSARCOMA CELLS.

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