0000000000923520

AUTHOR

Marc Lepetit

Membres du comité d’organisation

National audience

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Sélection pour l'architecture racinaire et les interactions symbiotiques chez le pois

National audience; La culture des légumineuses, et en particulier du pois, présente le double intérêt de permettre une production de graines à haute valeur nutritionnelle sans nécessité d’un apport d’engrais azoté et ainsi d’assurer à la fois sécurité alimentaire et respect de l’environnement. La nutrition azotée des légumineuses dépend en effet majoritairement de la fixation symbiotique de l’azote atmosphérique réalisée par des bactéries du sol, les rhizobia, au sein des nodosités, et dans une moindre mesure, de l’assimilation de l’azote minéral du sol par les racines. Cependant, la nutrition azotée du pois peut être insuffisante dans certains environnements et limiter ainsi le rendement d…

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Etude du déterminisme génétique pour le choix du partenaire symbiotique en vue d’améliorer la fixation d’azote chez le pois

Prod 2018-276 BAP GEAPSI INRA; National audience

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Déclaration d'invention et de résultats valorisables déposée par Marc Lepetit (LSTM INRA Montpellier) en mars 2018 pour dépôt de Licence d'exploitation exclusive sur savoir-faire (INRA-Transfert/LALLEMAND) 180205F "Souches de Rhizobium leguminosarum bv viciae P1NP1J et P221 pour l’inoculation de légumineuses". Contribution: Isolement d'une collection bactérienne et sélection au sein de cette collection d'une des 2 souches (P221) sur la base de caractérisations moléculaires et fonctionnelles

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Genetic diversity for partner choice in a core collection of pea accessions inoculated by a mix of five Rhizobium leguminosarum bv. viciae genotypes

National audience

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GRaSP project Genetics of Rhizobia Selection by Pea

Prod 2019-88aa; National audience; Because of their ability to fix nitrogen in symbiosis with soil bacteria, legumes such as peas have an important role to play in the development of a sustainable agriculture. However, in the field, biological nitrogen fixation (BNF) could be suboptimal as natural Rhizobium leguminosarum viciae (Rlv) populations are quantitatively and qualitatively heterogeneous, with strains varying in their competitiveness and efficiency of BNF [1,2]. There is a general agreement concerning the interest of Rlv inoculation for improving BNF and thus pea yield [3]. However, even when pea seeds are inoculated by efficient Rlv strains these can be outcompeted by indigenous rh…

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The competitiveness to form nodules shapes the capacities of Rhizobium leguminosarum sv viciae communities to promote symbiosis with specific hosts

National audience; Cultivated fabeae legumes (pea, fababean, lentil) develop root nodules resulting from the symbiotic interaction with Rhizobium leguminosarum sv. viciae (Rlv). Individual Rlv bacteria are able to associate with various potential hosts, but in soil they are in mixture and they display a wide range of competitiveness to form nodules (CFN). Because in Rlv, CFN and capacity to fix nitrogen are genetically independent, CFN limits the effectiveness of inoculation strategies as efficient bacteria are often outcompeted by poorly efficient Rlv bacteria of the soil community. We developed a strategy to identify bacterial genes controlling CFN. A worldwide collection of 240 Rlv isola…

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Root system architecture, nodulation and nitrogen nutrition in pea

National audience

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Genetic diversity of nodulated root structure in a very diverse pea collection

National audience; The root system is responsible for nitrogen (N) acquisition, which in legumes, combines mineral acquisition and symbiotic fixation in nodules. Despite these two complementary pathways, N nutrition may be a limiting factor of legumes yield because nodules are very sensitive to their local environment and N fixing legume root system is poorly developed which may limit soil exploration [1]. Pea establishes in root nodules a symbiotic association with Rhizobium leguminosarum sv viciae bacteria (Rlv) [2]. This study assessed the potential of naturally occurring genetic variability of nodulated root structure and functioning traits to improve yield pea performance. Two successi…

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