Affinity Distributions of a Molecularly Imprinted Polymer Calculated Numerically by the Expectation-Maximization Method

2003

Affinity distributions are calculated from adsorption isotherm data obtained for the enantiomers of L- and D-phenylalanine anilide (PA) on native and thermally annealed polymers molecularly imprinted with L-PA. The calculation is obtained with an iterative algorithm called expectation-maximization that does not require prior fit of the data to an isotherm model before inversion and thus yields a distribution indicative of the data only. The results show bimodal distributions, suggestive of a two-site model describing relatively selective and nonselective adsorption modes of the L-enantiomer and a corresponding unimodal/nonselective adsorption mode for the D-enantiomer. The nonselective adso…