0000000000928373
AUTHOR
Elvira Carissimi
showing 15 related works from this author
BRAFV600E mutation, TIMP-1 upregulation, and NF-κB activation: closing the loop on the papillary thyroid cancer trilogy.
2011
BRAFV600E is the most common mutation found in papillary thyroid carcinoma (PTC). Tissue inhibitor of metalloproteinases (TIMP-1) and nuclear factor (NF)-κB have been shown to play an important role in thyroid cancer. In particular, TIMP-1 binds its receptor CD63 on cell surface membrane and activates Akt signaling pathway, which is eventually responsible for its anti-apoptotic activity. The aim of our study was to evaluate whether interplay among these three factors exists and exerts a functional role in PTCs. To this purpose, 56 PTC specimens were analyzed for BRAFV600E mutation, TIMP-1 expression, and NF-κB activation. We found that BRAFV600E mutation occurs selectively in PTC nodules an…
In Vitro Phenotypic, Genomic and Proteomic Characterization of a Cytokine-Resistant Murine β-TC3 Cell Line
2012
Type 1 diabetes mellitus (T1DM) is caused by the selective destruction of insulin-producing β-cells. This process is mediated by cells of the immune system through release of nitric oxide, free radicals and pro-inflammatory cytokines, which induce a complex network of intracellular signalling cascades, eventually affecting the expression of genes involved in β-cell survival. The aim of our study was to investigate possible mechanisms of resistance to cytokine-induced β-cell death. To this purpose, we created a cytokine-resistant β-cell line (β-TC3R) by chronically treating the β-TC3 murine insulinoma cell line with IL-1β + IFN-γ. β-TC3R cells exhibited higher proliferation rate and resistan…
In Vitro Identification and Characterization of CD133pos Cancer Stem-Like Cells in Anaplastic Thyroid Carcinoma Cell Lines
2008
BackgroundRecent publications suggest that neoplastic initiation and growth are dependent on a small subset of cells, termed cancer stem cells (CSCs). Anaplastic Thyroid Carcinoma (ATC) is a very aggressive solid tumor with poor prognosis, characterized by high dedifferentiation. The existence of CSCs might account for the heterogeneity of ATC lesions. CD133 has been identified as a stem cell marker for normal and cancerous tissues, although its biological function remains unknown.Methodology/principal findingsATC cell lines ARO, KAT-4, KAT-18 and FRO were analyzed for CD133 expression. Flow cytometry showed CD133(pos) cells only in ARO and KAT-4 (64+/-9% and 57+/-12%, respectively). These …
Functional and molecular charecterizzation of cancer stam cells in anaplastic thyroid carcinoma
2011
Analisi dei meccanismi di controllo dell’apoptosi in una linea cellulare β-pancreatica resistente all’effetto delle citochine.
2008
BRAFV600E mutation and timp-1 hyper-expression in classical variants of papillary thyroid carcinoma (PTC).
2009
Identification and enrichment of thyroid stem cells in anaplastic cell lines
2008
Limbal stem cells as a potential source of pancreatic beta cells
2009
BACKGROUND/RATIONAL Beta cell replacement therapy represents an alternative approach to conventional insulin regimen for the management of type 1 diabetes. Stem cells theoretically represent a suitable and renewable source of surrogate beta cells. Recently, it has been demonstrated that corneal epithelium contains stem cells in the basal layer of the limbus. Our aim was to isolate and characterize limbal stem cells (LSCs) from adult corneal-limbal biopsies, and to assess their capability to differentiate into insulin-producing cells. METHODS 2-3 mm2 biopsies were obtained from limbus and central cornea of four patients undergoing surgery for ocular diseases not involving conjuctive or corne…
No association between BRAFV660E and metalloproteinase activity in anaplastic thyroid cell lines
2008
Molecular characterization of cancer stem cells in anaplastic thyroid carcinoma
2009
BRAFV660E mutation and Timp-1 hyper-expression in classical variants of papyllary thyroid carcinoma.
2009
BrafV600E mutation and hyperexpression of tissue inhibitor of metalloproteinases-1 (TIMP-1) in papillary thyroid carcinoma
2009
BRAFV600E MUTATION, TISSUE INHIBITOR OF METALLOPROTEINASE-1 UPREGULATION AND NF-KB ACTIVATION: CLOSING THE LOOP ON THE PAPILLARY THYROID CANCER TRILO…
2011
BRAFV600E is the most common mutation in papillary thyroid carcinoma (PTC). Tissue inhibitor of metalloproteinases (TIMP-1) and Nuclear Factor (NF)-kB have been shown to play an important role in thyroid cancer. Our aim was to evaluate whether an interplay among these three factors exerts a functional role in PTCs. 56 PTC specimens were analyzed for BRAFV600E mutation, TIMP-1 expression and NF-kB activation by real-time allele-specific amplification, realtime quantitative PCR (qRT-PCR) and electroforetic mobility shift assay (EMSA), respectively. We show that BRAFV600E mutation occurs selectively in PTC nodules and determines up-regulation of TIMP-1 and hyperactivation of NF-kB. In addition…