0000000000934402
AUTHOR
C. Olivas Arroyo
Kinetics in a Double Antibody Radioimmunoassay (RIA): Diffussion Control
Competitive protein binding radioimmunoassay (CPB-RIA) is a principal method for quantifying serum C Peptide concentration. The accuracy of this method is critically dependent on factors that influence the reaction between anti-C Peptide antibody (P) with 125I-C Peptide (M). We studied the influence of initial concentration of M, ionic strength, and viscosity on the reaction between M and P. A kinetic model for the the reaction between. Such model adjusts satisfac- torily to the results. Bi-exponential and irreversible kinetics is determined. The results of the viscosity analysis show clear negative influence on the direct reaction rate. The ionic strength shows scarce influence on equilibr…
Kinetics and equilibrium in insulin radioimmunoassay.
The kinetics of insulin reaction has been studied with its specific antibody immobilized on the inner wall of the reaction tube; the radioimmunoanalytical determination of such a substance is based on the reaction. Independent variables were labelled and unlabelled insulin concentrations, temperature, viscosity, and the medium's ionic strength. Biexponential kinetics was found to be dependent on the concentrations fitted to the models discussed in the paper. The effect of temperature shows activation parameters similar to the viscous flow energy of water, which suggests that the reaction is diffusion-controlled. The results of the viscosity analysis points at the clearly negative influence …