Analysis of gangliosides in biological samples by hydrophilic interaction liquid chromatography with electrospray ionization tandem mass spectrometry

International audience

Sample preparation and analysis of gangliosides and phospholipids from plasma by liquid chromatography coupled to mass spectrometry

Gangliosides (GG) and phospholipids (PL) are two classes of complex and polar lipids exhibiting a wide structural heterogeneity. LC/MS offers an access to this huge diversity but also faces some challenges. Efficient extraction and purification sample methods are thus required to perform accurate and reliable analyses. To this end, GG and PL were extracted from human plasma with three solvent mixtures (CHCl3/MeOH/H2O) of increasing polarity, in comparison with the reference Folch method. GG were further purified with a Phree Phospholipid Removal column, whose sorbent is designed to selectively remove PL. GG and PL classes were then separated under HILIC conditions before analysis using a Qq…

Rapport scientifique de l'ARVO

Gangliosides in the retina: a comparative inventory with brain and other ocular structures

International audience

Vulnerability of the nucleus accumbens neuronal network to developmental n-3 PUFA deficiency: consequences on the reward and motivation system

National audience; Various, though distinct psychiatric disorders, such as Schizophrenia, bipolar disorder or major depression are associated with a dysfunction of the reward system linked to an alteration of dopamine transmission. Furthermore, these pathologies are also accompanied by changes in lipid metabolism and in particular a decrease in the brain content n-3 polyunsaturated fatty acid (PUFA) in the nervous system. However, the implication of brain lipid composition in the etiology of psychiatric endophenotypes has been overlooked. The aim of this study was to investigate a potential causal link between n-3 PUFA deficiency and deficits in reward processing. Using operant conditioning…

24-(S)-hydroxycholesterol is a potent modulator of cholesterol metabolism in rat retinal Müller glia

International audience

Rapport scientifique de l'ARVO (Association for Research in Vision and Ophthalmology)

Modulation of lipid membrane organization of Müller Glial Cells (MGC) by 24-(S)-hydroxycholesterol (24-S-OHC) in glaucoma

Modulation of lipid membrane organization of Müller Glial Cells (MGC) by 24-(S)-hydroxycholesterol (24-S-OHC) in glaucoma. Session 3: Nutrition and oxysterols biological activities. ENOR Symposium

Characterization of cholesterol metabolism in an experimental model of glaucoma in rats

International audience; Glaucoma is a neurodegenerative pathology characterized by retinal ganglion cell death. Cholesterol homeostasis disruption has been implicated in several neurodegenerative diseases such as Alzheimer and Huntington. However, its specific role in the retina and association with glaucoma remain unclear. The aim of this study was to characterize the modifications of cholesterol metabolism occurring during an experimental model of glaucoma in rats. Eight-weeks old male rats were subjected to laser photocoagulation of episcleral veins, limbus and trabecular meshwork to elevate intraocular pressure. At a short (3 days) and a long (2 months) time period post-laser, the retin…

A monophasic extraction for simultaneous analysis of polar and non-polar lipids in brain sample by liquid chromatography – mass spectrometry

International audience