0000000000954129
AUTHOR
Karina Trindade
Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope
Lensless holographic microscope (LHM) is an emerging very promising technology that provides high-quality imaging and analysis of biological samples without utilizing any lens for imaging. Due to its small size and reduced price, LHM can be a very useful tool for the point-of-care diagnosis of diseases, sperm assessment, or microfluidics, among others, not only employed in advanced laboratories but also in poor and/or remote areas. Recently, several LHMs have been reported in the literature. However, complete characterization of their optical parameters remains not much presented yet. Hence, we present a complete analysis of the performance of a compact, reduced cost, and high-resolution LH…
Phase imaging microscopy under the Gabor regime in a minimally modified regular bright-field microscope
Quantitative phase imaging (QPI) is nowadays a powerful tool for visualization and analysis of biological processes. QPI is usually attained from specifically designed optical microscopes retrieving phase information in a quantitative way. In this paper we report on an extremely simple, low cost and compact way to update a standard bright-field microscope with coherent sensing capabilities. It is based on the in-line Gabor holography concept and only needs to replace the illumination broadband source of the regular microscope with a coherent one. The proposed methodology is completed by the recording of a digital in-line Gabor hologram instead of regular imaging conditions and by the numeri…