Effects of endocrine disruptors on genes associated with 17 beta-estradiol metabolism and excretion

International audience; In order to provide a global analysis of the effects of endocrine disruptors on the hormone cellular bioavailability, we combined 17 beta-estradiol (E2) cellular flow studies with real-time PCR and Western blot expression measurements of genes involved in the hormone metabolism and excretion. Three endocrine disruptors commonly found in food were chosen for this study, which was conducted in the estrogen receptor (ER) negative hepatoblastoma HepG2 cell line: bisphenol A (BPA), genistein (GEN) and resveratrol (RES). We showed that 24h after a single dose treatment with genistein, resveratrol or bisphenol A, the expression of ATP-binding cassette transporters (the mult…

Endocrine disruptors found in food modulate estradiol cellular metabolism

Major sulfation pathway for resveratrol metabolism in human hepatic derived cells and resveratrol –dependent inducibility of conjugating enzymes. Perspective in nutritional prevention

Endocrine disruptors found in food modulate estradiol cellular metabolism

Major sulfation pathway for resveratrol metabolism in human hepatic derived cells and resveratrol –dependent inducibility of conjugating enzymes. Perspective in nutritional prevention