0000000000976760
AUTHOR
Julia Buitink
showing 12 related works from this author
Use of translational genomics to identify genes important for legume seed filling
2013
International audience; Translational genomics, i.e., the transfer of genetic information from model species to cultivated crops, is on the brink of revolutionizing plant breeding. The recent publication of genomic sequences for several cultivated legumes is also accelerating this process. For pea, recent highthroughput RNA sequencing, and the prospect of a genome sequencing project, will further accelerate the transfer of information from the Medicago truncatula model to the cultivated crop. We have been using genomics approaches with Medicago as a tool to identify key genes determining seed yield and composition in closely related legumes. Analyses of the proteome and transcriptome of the…
Use of translational genomics to identify genes important for legume seed development
2015
BAP Pôle GEAPSI; International audience; We have exploited the extensive synteny between the model legume Medicago truncatula and the cultivated garden pea, Pisumsativum, to identify loci controlling seed filling and seedcomposition in the crop species. QTLs for these traits are mapped by analyzing variation with in collections of recombinant inbred lines. Candidate genes with in the QTL intervals are identified by reference to the M.truncatula genomic sequence. This approach was used to uncover an endosperm subtilase that is associated with syntenic seed weight QTLs in Medicago and pea, and we discuss the possible role played by this enzyme in contributing to final seed weight.
The role of sulfur in the control of seed quality traits in legumes: from model to crop species
2017
National audience; voir pdf
Importance des voies de recyclage de la méthionine dans le contrôle de la plasticité de la composition protéique des graines
2021
International audience; La production de protéines végétales en France et en Europe nécessite de fournir des outils d’aide à la sélection de variétés de légumineuses produisant des graines de meilleure valeur nutritionnelle possible. Bien que certains constituants réputés antinutritionnels aient été réduits dans les graines des variétés de légumineuses, la fraction protéique reste à améliorer et à stabiliser pour d’avantage d’équilibre en acides aminés. Cet enjeu nécessite d’identifier les gènes contrôlant la composition protéique des graines et sa plasticité vis-à-vis de l’environnement. Une approche de génétique d’association à l’échelle du génome (GWAS) appliquée à 200 écotypes de Medica…
Recherche des gènes contrôlant la plasticité de la composition protéique des graines vis-à-vis de l’environnement par une approche GWAS chez Medicago…
2019
National audience; Les légumineuses sont capables de produire des graines riches en protéines en l’absence d’apports azotés grâce aux symbioses qu’elles établissent avec des bactéries fixatrices d’azote de l’air. Riches en lysine, ces protéines sont utilisées pour la nutrition humaine et l’alimentation animale. Cependant, les instabilités de la teneur et de la composition protéique des graines causées par les variations environnementales limitent l’adoption des légumineuses en Europe. Afin de promouvoir leur culture, il est nécessaire d’optimiser et de stabiliser la quantité et la qualité de cette fraction protéique. Nous avons étudié la plasticité de la teneur et de la composition protéiqu…
Genetic determinants of seed protein plasticity in response to the environment in Medicago truncatula
2021
As the frequency of extreme environmental events is expected to increase with climate change, identifying candidate genes for stabilizing the protein composition of legume seeds or optimizing this in a given environment is increasingly important. To elucidate the genetic determinants of seed protein plasticity, major seed proteins from 200 ecotypes of Medicago truncatula grown in four contrasting environments were quantified after one-dimensional electrophoresis. The plasticity index of these proteins was recorded for each genotype as the slope of Finlay and Wilkinson's regression and then used for genome-wide association studies (GWASs), enabling the identification of candidate genes for d…
Elucidating the genetic determinism of the plasticity of seed proteins in response to the environment using Medicago truncatula
2019
International audience; Legumes are able to produce high-protein seeds without nitrogen fertilizer through root symbiosis with nitrogen-fixing rhizobia. Rich in lysine, these proteins are used for human nutrition and animal feed. However, instability of seed protein yield and quality due to environmental fluctuations limits the wide adoption of legumes in Europe. Breeding efforts are needed to optimize and stabilize seed nutritional quality. We have studied the plasticity of protein content and composition of seeds from a collection of 200 ecotypes of Medicago truncatula grown under four controlled conditions (optimal, drought, and winter/spring sowing). A quantitative analysis of one-dimen…
2000-2009 Publication metrics reveal world trends of grain legume research
2012
Identifiant ProdInra 271566 BAP SAR CICAP GEAPSI pas de classement CT scientifiqueIdentifiant ProdInra 271566BAPSAR CICAPGEAPSIpas de classement CT scientifique; Grain legumes grown in Europe produce protein-rich seeds of good nutritional value. They can partially replace imported soybean amounting to about 35 Mt in 2010. Legumes also fix N2 naturally with the help of rhizobiaceae bacteria, resulting in fossil energy saving and reduction of glasshouse gas emissions risks. Strength and weaknesses of grain legume research in Europe and other nations are not well known. Therefore we studied 2000-2009 worldwide publications on grain legumes using the CAB database. We built a world corpus of 161…
The roles of the embryo-surrounding tissues in regulating Medicago truncatula seed filling
2011
National audience
Regulation of the plasticity of longevity upon drought in Medicago truncatula involves the cryptochrome-interacting bHLH49 transcription factor
2021
International audience
Déterminisme génétique de la plasticité de la composition protéique des graines de légumineuses vis-à-vis de l'environnement
2018
National audience; voir pdf
Legume adaptation to sulfur deficiency
2014
BAPSPEEAGEAPSIIPM; Because sulfur-deficiency represents an agronomic issue for crops and notably for the quality of legume products, it is necessary to provide sulfur fertilizers according to crop sulfur needs along with improving sulfur use efficiency. To achieve this goal, increased knowledge of how plants respond to sulfur-deficiency is required. We subjected Medicago truncatula, a model legume species, to sulfur deficiency at different developmental stages and compared yield, nutrient allocation and seed traits. We revealed an impressive plasticity of M. truncatula that varied according to the S-stress period and influenced seed composition and germination. Sulfur deficiency at a mid-ve…