0000000000982533

AUTHOR

Carlos Gilberto Carlotti

showing 1 related works from this author

Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR

2009

Abstract Background Considering the broad variation in the expression of housekeeping genes among tissues and experimental situations, studies using quantitative RT-PCR require strict definition of adequate endogenous controls. For glioblastoma, the most common type of tumor in the central nervous system, there was no previous report regarding this issue. Results Here we show that amongst seven frequently used housekeeping genes TBP and HPRT1 are adequate references for glioblastoma gene expression analysis. Evaluation of the expression levels of 12 target genes utilizing different endogenous controls revealed that the normalization method applied might introduce errors in the estimation of…

Hypoxanthine PhosphoribosyltransferaseCell typeLung Neoplasmslcsh:QH426-470Journal ClubCellGene ExpressionComputational biologyBiologyBioinformaticsModels BiologicalVariable ExpressionReference genesExpression analysisGene expressionmedicineHumansStudent’s Sectionlcsh:QH573-671Molecular BiologyGeneSelection (genetic algorithm)GeneticsRegulation of gene expressionGenes Essentiallcsh:CytologyBrain NeoplasmsReverse Transcriptase Polymerase Chain ReactionMethodology ArticleGeneral NeuroscienceReference StandardsTATA-Box Binding Proteinmedicine.diseaseHousekeeping geneDNA-Binding ProteinsGene Expression Regulation Neoplasticlcsh:GeneticsNEOPLASIAS DO SISTEMA NERVOSOReal-time polymerase chain reactionmedicine.anatomical_structureGlioblastomaGlioblastomaAnnals of Neurosciences
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