0000000001009846

AUTHOR

Demetrios A. Spandidos

0000-0002-1146-931x

showing 1 related works from this author

Intercellular communication and human hepatocellular carcinoma.

2005

We have previously reported that gap junction-mediated intercellular communication (GJIC) can be restored in junctionally deficient human prostate epithelial cells, also suggesting that GJIC activity is regulated by estrogen. In the present work, we report studies on sex steroid regulation of GJIC and proliferative activity in both nontumoral (Chang liver, CL) and malignant (HepG2, Huh7) human liver cells. Junctional activity and liver cell growth were measured using the scrape-loading/dye-transfer (SL/DT) and the MTS assay, respectively. Using the SL/DT, only Huh7 cells exhibited a moderate degree of Junctional activity in basic conditions, while neither CL nor HepG2 cells showed functiona…

Receptors SteroidTime FactorsProliferationCell Communicationchemistry.chemical_compoundNeoplasmsReceptorTumorGeneral NeuroscienceLiver cellLiver NeoplasmsGap JunctionsGap junction-mediated intercellular communication (GJIC)ImmunohistochemistryLiverLiver NeoplasmReceptors AndrogenGap JunctionReceptors ProgesteroneHumanmedicine.medical_specialtyCell signalingCarcinoma HepatocellularTime Factormedicine.drug_classEstroneBiologyGeneral Biochemistry Genetics and Molecular BiologyCell LineHistory and Philosophy of ScienceInternal medicineCell Line TumormedicineCarcinomaEstrogen Receptor betaHumansHepatocellular carcinoma (HCC)SteroidCell ProliferationBiochemistry Genetics and Molecular Biology (all)Cell growthEstrogen Receptor alphamedicine.diseasedigestive system diseasesEndocrinologychemistryEstrogenCell cultureCancer researchNeoplasmAnnals of the New York Academy of Sciences
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