0000000001011675
AUTHOR
C. Boulin
Resonant X-Ray Scattering of Biological Systems
Nearly all synchrotron radiation laboratories at high energy electron storage rings put enormous effort into the developement of resonant (anomalous) X-ray scattering techniques. So does the Hamburger Synchrotronstrahlungslabor (HASYLAB) at Hamburg. One of these intruments which is built in cooperation with the university of Mainz at the beam line A1 of the storage ring DORIS. How does the physics of resonant scattering enter into the design of the X-ray instrument.
Advances in contrast variation for macromolecular structure determination by polarized neutron scattering and anomalous dispersion of synchrotron X-rays
Contrast variation for macromolecular structure determination is usually achieved by isomorphous replacement of 1-H by 2-H (D) using small-angle neutron scattering (SANS). This is particularly easy in aqueous solvents. By adding heavy water the contrast of dissolved proteins, nucleic acids and membranes changes drastically. It is the region inaccesible to solvent molecules, which acts as a label. Measurements of the scattering intensity at three different scattering densities of a solvent yields the three basic scattering functions. The contrast dependence of the radius of gyration receives particular interest. More recently smaller labels have been used. Their dimensions are smaller than t…