0000000001018270
AUTHOR
P. Cordioli
showing 4 related works from this author
Valutazione di alcune performance diagnostiche di kit ELISA per la diagnosi sierologica di Anemia Infettiva Equina (AIE)
2012
Data on evaluation of some diagnostic parameters of all ELISA kits available in Italy for the serodiagnosis of AIE are presented and discussed. Ten laboratories were involved using a panel of 30 sera ran with 4 commercial kits and 2 in-house kits. Kits were also evaluated using a panel of sera from vaccinated animals at different days post vaccination (p.v.). All sera were also tested in agar gel immunodiffusion (AGID). The parameters evaluated were: diagnostic sensitivity (DSe) and specificity (DSp), Cohen K, weighted Cohen K, coefficient of variation (CV), accordance, concordance. Analysis of these parameters indicates that all kits have a higher sensitivity than AGID, even if a complete …
Pandemic influenza A/H1N1 virus in a swine farm house in Sicily, Italy.
2012
This report describes a pandemic A/H1N1 (H1N1 pdm) virus outbreak occurred in December, 2009 in a swine farm used as research facility (Istituto Mediterraneo Trapianti e Terapie ad Alta Specializzazione) for preclinical studies, located in Sicily, Italy. All the 13 pigs of the farm, showed cough, fever, inappetence and weakness. At the same time, an unvaccinated worker of the stabling showed influenza-like symptoms. RNAv extracted from two swabs collected from infected pigs resulted positive by Real Time RT-PCR for Influenza A virus. Furthermore, after growth on embryonated eggs, viral isolates were identified by Real Time RT-PCR specific for H1N1 pdm virus and characterized antigenically. …
Virus influenzali H1N1pdm e riassortanti isolati dal suino
2011
Sin dai primi giorni della circolazione del virus pandemico H1N1-2009 nella popolazione, è stata segnalata la trasmissione del virus dall’uomo a specie animali diverse (suino, tacchino, cane, gatto). La trasmissione di virus influenzali dall’uomo agli animali non rappresenta solo un problema limitato al settore zootecnico, ma un problema di sanità pubblica per il ruolo di “serbatoio” dei virus influenzali rappresentato da tali specie. Il suino non ha sinora dimostrato un ruolo epidemiologico importante nella diffusione di (H1N1)pdm all’uomo; tuttavia ciò non esclude, che a seguito dell’evoluzione virale, o di fenomeni di riassortimento, da tale specie emergano e si trasmettano all’uomo viru…
Rapid sequencing and genetic analysis of the pandemic (H1N1)V influenza virus circulating in pigs in Italy
2011
will continue to pose challenge to public health and scientifi c community. General concern exists about possible mutation or reassortment between the H1N1pdm and infl uenza viruses circulating in human and animal, giving rise to more transmissible or pathogenic viruses. Emergence of resistance during antiviral treatment is a wellrecognised phenomenon in infl uenza viruses; surveillance for emergence of resistant viruses is of importance for monitoring this potential public health problem in the context of the H1N1 pandemic. Thus preparedness to identify new strains would require fast sequencing of the full genome of virus. Here we present an optimised workfl ow for rapid sequencing of the …