0000000001024890

AUTHOR

Isaías Balderas-rentería

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Transcriptional Upregulation of Genes Related to Virulence Activation in Entamoeba histolytica

2007

Background To understand the molecular basis of virulence variability in Entamoeba histolytica , this study presents results about differential gene expression induced by E. histolytica trophozoites in liver of hamsters in order to produce experimental amebic liver abscess (ALA) and consequently reactivate its virulence. Methods Amebic cultures were studied before (BALA) and after (AALA) inoculation in hamster peritoneal cavity. Markers of pathogenicity such as the rate of erythrophagocytosis, hemolytic activity, and cytotoxic effects on MDCK cell monolayers were evaluated in order to correlate these phenotypic characteristics to differential gene expression between virulent and non-virulen…

VirulencePolymerase Chain ReactionMicrobiologyEntamoeba histolyticaCricetinaeparasitic diseasesGene expressionAnimalsRNA MessengerTrophozoitesGeneRegulation of gene expressionPolymorphism GeneticVirulencebiologyGene Expression ProfilingEntamoeba histolyticaGeneral MedicineRibosomal RNAbiology.organism_classificationMolecular biologyUp-RegulationGene expression profilingPhenotypeGene Expression RegulationLiverFunctional genomicsArchives of Medical Research
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