Caracterisation of the mecanisms regulating membrane structuration modifications induced during defense response signalisation in plants

Spatial distribution of pasma membranes (PM) components is tightly regulated to provide the cell an optimal physiological state. The level of order degree is a suitable parameter to study PM organization, reflecting the intensity of interactions taking place between PM components and so the level of their packing. During our work, we used the environment sensitive probe di-4-ANEPPDHQ to assess the level of order degree of tobacco BY-2 cells PM in different situations: during the time-course of cell regeneration and in the particular case of an elicitation by cryptogein.We measured that the level of order degree of PM is modulated during the slow process of cell regeneration. This regulation…

Participation à la fête de la nature et de la biodiversité au Jardin des Sciences du Parc de l’Arquebuse à Dijon le dimanche 21 mai 2017

Plant lipids: Key players of plasma membrane organization and function

International audience; The Plasma Membrane (PM) is a key structure protecting the cell, regulating nutrient exchanges and acting as a control tower allowing the cell to perceive signals. Plasma comes from the greek πλάσμα meaning "which molds", meaning that the PM takes the shape of the cell by delimitating it. The PM harbors the appropriate signaling cascades allowing adaptive responses ensuring proper cell functions in a continuously fluctuating environment, crucial for cell survival. To address this challenge, the PM needs to be both stable and robust yet incredibly fluid and adaptable. This amazing combination of long-term stability and short-term dynamics in order to adapt to signals …

Exocytosis and endocytosis act together to modify the plasma membrane order in cryptogein-elicited tobacco cells

SPEIPMUBINRA; Exocytosis and endocytosis act together to modify the plasma membrane order in cryptogein-elicited tobacco cells. 19. European Network for Plant Endomembrane Research meeting

Characterization of membrane structure modifications induced during plant defense signaling

SPEIPMUBINRADOCT; Plasma membrane was long considered as a simple separation between internal and external cellular environment. But more, it is a dynamic interface, an exchange platform. Now, recent researches highlight its role in plant defense responses to biotic and abiotic stresses. We use the tobaccocryptogein model to study the early steps of plant cell responses to pathogen detection. The signaling cascade includes an oxidative burst triggering an increase of plasma membrane order. The level of membrane order informs us about both packing and arrangement of membrane components, this parameter being evaluated by spectrofluorimetric approaches using a lipidic environment-sensitive pro…

Analyse de la signalisation précoce de cellules.