Effect of cadmium on growth, isoflavonoid and protein accumulation patterns in mycorrhizal roots of Medicago truncatula

International audience; Ecosystems are submitted to various abiotic stresses, among which heavy metals represent major industrial pollutants. Cadmium (Cd), that has damaging effects on plant metabolism, occurs in agricultural environments through industrial pollution and human activities, including phosphate fertiliser and sewage sludge applications. Metal availability to plants can be modulated by soil microorganisms, including arbuscular mycorrhizal (AM) fungi. In the present work, Cd effects on the model legume Medicago truncatula inoculated or not with the AM fungus Glomus intraradices have been studied at 3 levels: (1) plant biomass production together with green part chlorophyll quant…

Germination and storage protein mobilisation of Trifolium subterraneum Var 45C seeds under hypoxic treatment

International audience; The rate, speed and latency period of germination were defined for seeds of Trifolium subterraneum variety 45 C. The latency period was 12 hours on distilled water. The hypoxic stress was applied at the beginning (T12) and the end of latency period (T24). It consists on the addition of nitrogen (N2) under 1 bar pressure in hermetically closed boxes during 2 hours. The germination of treated seeds was then assessed on distilled water. The germination test during hypoxic treatment showed that the hypoxic stress delayed germination, slowing down its speed and decreasing the final percentage of germinated seeds. In order to better understand this depressive effect observ…

Cadmium stress alleviation by arbuscular mycorrhizal symbiosis: proteomic studies of the mechanisms involved in shoots of M. truncatula