Oil-degrading bacteria from a membrane bioreactor (BF-MBR) system for treatment of saline oily waste: Isolation, identification and characterization of the biotechnological potential
A collection of forty-two (42) strains was obtained during microbiological screening of a Membrane Bioreactor (MBR) system developed for the treatment of saline oily waste originated from marine transportation. The diversity of the bacterial collection was analyzed by amplification and sequencing of the 16S rRNA gene. Taxonomic analysis showed high level of identity with recognized sequences of seven (7) distinct bacterial genera (Alcanivorax, Erythrobacter, Marinobacter, Microbacterium, Muricauda, Rhodococcus and Rheinheimera). The biotechnological potential of the isolates was screened considering an important factor such as the biosurfactant production. In particular fourteen (14) biosur…
Isolation and Characterization of Oil-Degrading Bacteria from Bilge Water
Twenty-one oil-degrading bacteria were isolated from bilge water. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 7 strains were selected (from 21 isolated) for further studies. 16S rRNA gene sequencing showed that isolated strains were affiliated to Bacillus, Pseudomonas and Halomonas genera; in particular, isolate BW-B12 (Bacillus sp., 99%), BW-C12 (Halomonas boliviensis, 99%) and BW-E12 (Halomonas boliviensis, 98%) were the best crude-oil degraders; after 10 days of cultivation in ONR 7a mineral medium supplemented with crude oil as single carbon source BW-B12, BW-C12 and BW-E12 showed a degradation rate of 80, 60 and 59%, respectively. The strains showed…