0000000001067245
AUTHOR
María ÁNgeles Goberna-bravo
Determination of isoniazid and pyridoxine in plasma sample of tuberculosis patients by micellar liquid chromatography
It is no doubt Isoniazid is a powerful tuberculosis drug, but it might give rise to Vitamin B6 (Pyridoxine) deficiency. In this case, a usual treatment is the combined administration of Isoniazid and Pyridoxine. An easy-to-conduct procedure based on Micellar Liquid Chromatography has been developed to quantify Isoniazid and Pyridoxine in plasma from Tuberculosis patients. The sample was diluted in mobile phase, filtered and directly injected, thus avoiding extraction or purification steps. Both drugs were adequately resolved from the matrix and endogenous compounds using a mobile phase made up of 0.15 M sodium dodecyl sulfate – 8%(v/v) 1-butanol – 0.01 M phosphate buffer at pH 3, running at…
Screening of some banned aromatic amines in textile products from Indian bandhani and gamthi fabric and in human sweat using micellar liquid chromatography
Certain dyes in textile products, which are capable of reductively splitting into carcinogenic aromatic amines, are strictly controlled in many countries. A simple, rapid, sensitive and green chromatographic method has been developed and validated for the simultaneous determination of 4-aminophenol (4-AMP), p-phenylenediamine (p-PPD) and benzidine (BNZ), banned aromatic amines in dyeing clothes and human sweat. The separation was achieved using a micellar mobile phase of 0.1 M SDS, 4% 1- butanol (v/v) buffered to pH 7 with sodium dihydrogen phosphate, flowing under isocratic mode at 1 mL/min through a C18 column. Photodiode array detector was set at 210 nm. Using the above chromatographic c…