0000000001073450

AUTHOR

Robin W. Briehl

showing 2 related works from this author

Contributory presentations/posters

1999

0303 health sciencesbiologyGeneral Medicine010402 general chemistry01 natural sciencesHorseradish peroxidaseGeneral Biochemistry Genetics and Molecular Biology0104 chemical sciences03 medical and health sciencesBiochemistryManganese porphyrinbiology.proteinEnzyme reconstitutionGeneral Agricultural and Biological Sciences030304 developmental biologyJournal of Biosciences
researchProduct

Light Scattering Measurements of Hemoglobin Critical Fluctuation and the Energy Landscape For Polymerization

2010

We have developed a novel method for measuring light scattering to observe critical fluctuations in hemoglobin (Hb) solutions. A small rectangular cell (0.2 x 4.0 x 30 mm) is filled with 24 μL of Hb solution. An optical fiber with outer diameter of 125 μm (62.5 μm core) is sealed into the cell in contact with the solution, and light scattering is measured at 90°. The flat faces of the cell permit measuring absorbance spectra to ensure sample integrity. The scattering source is a 785 nm laser diode that delivers 1.5 mW to the sample. Scattered light is detected by a Hamamatsu GaAs(Cs) PMT via a LWD microscope objective. Measured scattered light intensity agrees (±10%) with scattered intensit…

SpinodalOptical fiberLaser diodeScatteringChemistryAnalytical chemistryBiophysicsMolecular physicsLight scatteringIntensity (physics)law.inventionCore (optical fiber)PolymerizationlawBiophysical Journal
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