0000000001074566

AUTHOR

J. Mir

showing 2 related works from this author

Effects of steatosis on drug-metabolizing capability of primary human hepatocytes.

2007

Abstract The suitability of liver grafts discarded for transplantation because of macrosteatosis for preparing human hepatocyte cultures for in vitro drug metabolism studies has been examined. Lower cell viability and yield of isolation procedure were obtained from fatty livers (>40% steatosis) with respect to normal tissue. Significant reductions in 7-ethoxycoumarin O-deethylation (ECOD) and testosterone oxidations were found in hepatocytes prepared from steatotic livers. The potential impact of lipid accumulation on P450 enzymes was studied in vitro by incubation of cultured hepatocytes with long chain free fatty acids (FFA). Treatment of cells with 0.25–3 mM FFA induced dose-dependent ac…

medicine.medical_specialtyCYP3A4CYP1A2General MedicineBiologyCYP2E1Fatty Acids NonesterifiedToxicologymedicine.diseaseTransplantationFatty LiverEndocrinologymedicine.anatomical_structureCytochrome P-450 Enzyme SystemInternal medicineHepatocytemedicineHepatocytesHumansTestosteroneViability assaySteatosisOxidation-ReductionDrug metabolismCells CulturedToxicology in vitro : an international journal published in association with BIBRA
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Human Hepatocyte Transplantation in Patients with Hepatic Failure Awaiting a Graft

2012

<b><i>Background:</i></b> Hepatocyte transplantation (HT) has the potential to become a promising treatment to temporarily support liver function in patients with liver failure. <b><i>Methods:</i></b> Two patients, who had already received a liver transplant (LT) in the past, with an end-stage liver disease due to recurrent hepatitis C virus cirrhosis, suffering acute-on-chronic liver failure while on the waiting list for an LT, received HT as a bridge to whole-organ retransplantation. After HT and during intensive care unit admission, blood tests and ammonia levels were determined every 12 and 24 h, respectively, before and after each hepatoc…

AdultMaleReoperationmedicine.medical_specialtyTime FactorsWaiting Listsmedicine.medical_treatmentLiver transplantationChronic liver diseaseGastroenterologyEnd Stage Liver DiseaseHepatocyte transplantationLiver Function TestsAmmoniaInternal medicinemedicineHumansIn patientmedicine.diagnostic_testbusiness.industryHepatitis CHepatitis C ChronicMiddle Agedmedicine.diseaseLiver TransplantationTransplantationHepatocytesSurgeryLiver functionbusinessLiver function testsEuropean Surgical Research
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