0000000001107785

AUTHOR

Jean-marc Lebel

showing 3 related works from this author

The shell matrix of the pulmonate land snail Helix aspersa maxima.

2012

12 pages; International audience; In mollusks, the shell mineralization process is controlled by an array of proteins, glycoproteins and polysaccharides that collectively constitute the shell matrix. In spite of numerous researches, the shell protein content of a limited number of model species has been investigated. This paper presents biochemical data on the common edible land snail Helix aspersa maxima, a model organism for ecotoxicological purposes, which has however been poorly investigated from a biomineralization viewpoint. The shell matrix of this species was extracted and analyzed biochemically for functional in vitro inhibition assay, for amino acid and monosaccharides composition…

0106 biological sciencesBiomineralizationPulmonate snailPhysiology01 natural sciencesBiochemistryMineralization (biology)chemistry.chemical_compoundX-Ray DiffractionTandem Mass SpectrometryElectrophoresis Gel Two-DimensionalHaliotisAmino AcidsComputingMilieux_MISCELLANEOUSchemistry.chemical_classification0303 health sciencesEcologyMonosaccharidesLand snailImmunogold labellingImmunohistochemistryAmino acidBiochemistryElectrophoresis Polyacrylamide GelTerrestrial snail ; biomineralization ; shell ; aragonite ; crossed-lamellar ; protein ; immunogold ; gel electrophoresisFrancefood.ingredientBiology010603 evolutionary biologyCalcium Carbonate03 medical and health sciencesfoodSpecies SpecificityAnimal ShellsShellAnimals14. Life underwater[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsMolecular Biology030304 developmental biologyHelix SnailsProteinsCrossed-lamellarbiology.organism_classification[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/BiomaterialsGel electrophoresis[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate ZoologyCalcium carbonatechemistryMicroscopy Electron ScanningBiomineralizationPinctadaComparative biochemistry and physiology. Part B, Biochemistrymolecular biology
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Shell repair process in the green ormer Haliotis tuberculata: a histological and microstructural study.

2008

In the present paper, juvenile and adult shells of the green ormer Haliotis tuberculata ('Oreille de Saint-Pierre') were perforated in a zone close to the shell edge and the shell repair process was followed at two levels: (1) by observing the histology of the calcifying mantle in the repair zone and (2) by analyzing with SEM the microstructure of the shell repair zone. Histological data clearly show the presence of calcium carbonate granules into the connective tissues, but not in the epithelial cells. This suggests that calcium carbonate granules are synthesized by sub-epithelial cells and actively transported through the epithelium to the repair zone, via a process which may be similar t…

0106 biological sciencesBiomineralizationfood.ingredient010603 evolutionary biology01 natural sciencesMineralization (biology)Epithelium03 medical and health scienceschemistry.chemical_compoundfoodMantle epitheliumGreen ormerAnimalsShell repair14. Life underwaterHaliotisPinctadaMantle (mollusc)[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsGastropodMicrostructureComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesWound HealingbiologyCell BiologyGeneral MedicineAnatomybiology.organism_classification[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterials[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate ZoologyKineticsCalcium carbonatechemistryBiophysicsMicroscopy Electron ScanningEastern oysterCalcium carbonateDevelopmental BiologyPinctadaBiomineralization
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Shell Extracts from the Marine Bivalve Pecten maximus Regulate the Synthesis of Extracellular Matrix in Primary Cultured Human Skin Fibroblasts

2014

International audience; Mollusc shells are composed of more than 95% calcium carbonate and less than 5% of an organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. Previous studies have elucidated the biological activities of the shell matrices from bivalve molluscs on skin, especially on the expression of the extracellular matrix components of fibroblasts. In this work, we have investigated the potential biological activities of shell matrix components extracted from the shell of the scallop Pecten maximus on human fibroblasts in primary culture. Firstly, we demonstrated that shell matrix components had different effects on general cellular activities. Secondly, …

Pathologylcsh:Medicine[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC][CHIM.THER]Chemical Sciences/Medicinal ChemistryBiochemistryExtracellular matrixCell SignalingMolecular Cell Biologylcsh:ScienceSkinConnective Tissue Cellschemistry.chemical_classificationPectenMultidisciplinary[ CHIM.THER ] Chemical Sciences/Medicinal ChemistryExtracellular MatrixCell biologymedicine.anatomical_structureConnective TissueScallopCytochemistryAnatomyCellular Structures and OrganellesType I collagenResearch ArticleBiotechnologySignal Transductionmedicine.medical_specialtyPrimary Cell CultureExtracellular Matrix SignalingBiologyBiomaterialsDermisAnimal ShellsmedicineAnimalsHumansPecten maximus14. Life underwaterTissue Extractslcsh:R[ SDV.BC.BC ] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]Biology and Life SciencesCell BiologyFibroblastsbiology.organism_classificationIn vitroBiological TissuechemistryCell culturelcsh:QGlycoprotein
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