0000000001115508

AUTHOR

Cristina Escarmís

showing 2 related works from this author

Evidence for Positive Selection in the Capsid Protein-Coding Region of the Foot-and-Mouth Disease Virus (FMDV) Subjected to Experimental Passage Regi…

2001

We present sequence data from two genomic regions of foot-and-mouth disease virus (FMDV) subjected to several experimental passage regimens. Maximum-likelihood estimates of the nonsynonymous-to-synonymous rate ratio parameter (dN/dS) suggested the action of positive selection on some antigenic sites of the FMDV capsid during some experimental passages. These antigenic sites showed an accumulation of convergent amino acid replacements during massive serial cytolytic passages and also in persistent infections of FMDV in cell culture. This accumulation was most significant at the antigenic site A (the G-H loop of capsid VP1), which includes an Arg-Gly-Asp (RGD) cellular recognition motif. Our …

parallel evolutionmedicine.drug_class[SDV]Life Sciences [q-bio]virusesMolecular Sequence DataPopulationMonoclonal antibodyVirusEvolution Molecular03 medical and health sciencesAphthovirusCapsidAntigenpositive selectionGeneticsmedicineCoding regionSelection GeneticSerial Passageconvergent evolutioneducationMolecular BiologyPhylogenyEcology Evolution Behavior and Systematics030304 developmental biologychemistry.chemical_classification0303 health scienceseducation.field_of_studyModels GeneticbiologyFoot-and-mouth disease virusfoot-and-mouth disease virusexperimental phylogeny030306 microbiologyParallel evolutionbiochemical phenomena metabolism and nutritionbiology.organism_classificationVirology3. Good healthAmino acidPositive selection[SDV] Life Sciences [q-bio]CapsidchemistryFoot-and-mouth disease virusExperimental phylogenyConvergent evolutionMolecular Biology and Evolution
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Genetic lesions associated with Muller's ratchet in an RNA virus

1996

The molecular basis of Muller's ratchet has been investigated using the important animal pathogen foot-and-mouth disease virus (FMDV). Clones from two FMDV populations were subjected to serial plaque transfers (repeated bottleneck events) on host BHK-21 cells. Relative fitness losses were documented in 11 out of 19 clones tested. Small fitness gains were observed in three clones. One viral clone attained an extremely low plating efficiency, suggesting that accumulation of deleterious mutations had driven the virus near extinction. Nucleotide sequence analysis revealed unique genetic lesions in multiply transferred clones that had never been seen in FMDVs isolated in nature or subjected to m…

Mutational hot spotvirusesViral quasispeciesViral Plaque AssayVirusOligoadenylate extensionCell LineExtinction PsychologicalAphthovirusStructural BiologyCricetinaePolyadenylateAnimalsFitness lossMolecular BiologyGeneticsbiologyFoot-and-mouth disease virusNucleic acid sequenceRNA virusMuller's ratchetbiology.organism_classificationVirologyQuasispeciesCapsidMutationNucleic Acid ConformationRNA ViralFoot-and-mouth disease virus
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