0000000001120338

AUTHOR

Yet-ran Chen

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Induction of cholesterol biosynthesis by archazolid B in T24 bladder cancer cells.

2014

Abstract Background Resistance of cancer cells towards chemotherapeutics represents a major cause of therapy failure. The objective of our study was to evaluate cellular defense strategies in response to the novel vacuolar H+-ATPase inhibitor, archazolid B. Experimental approach: The effects of archazolid B on T24 bladder carcinoma cells were investigated by combining “omics” technologies (transcriptomics (mRNA and miRNA) and proteomics). Free cholesterol distribution was determined by filipin staining using flow cytometry and fluorescence microscopy. Flow cytometry was performed for LDLR surface expression studies. Uptake of LDL cholesterol was visualized by confocal microscopy. SREBP acti…

IndolesCell SurvivalBiologyReal-Time Polymerase Chain ReactionBiochemistryFatty Acids Monounsaturatedchemistry.chemical_compoundCell Line TumormedicineHumansFluvastatinPharmacologyCholesterolReproducibility of ResultsMolecular biologySterolEndocytosisSterol regulatory element-binding proteinGene Expression Regulation NeoplasticLipoproteins LDLMicroRNAsThiazolesCell killingCholesterolchemistryReceptors LDLUrinary Bladder NeoplasmsDrug Resistance NeoplasmLDL receptorCancer celllipids (amino acids peptides and proteins)Sterol regulatory element-binding protein 2MacrolidesSterol Regulatory Element Binding Protein 1Fluvastatinmedicine.drugSterol Regulatory Element Binding Protein 2Biochemical pharmacology
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