0000000001133326

AUTHOR

Bertram Brenig

showing 2 related works from this author

2004

Background As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species.

GeneticsCytochrome b010401 analytical chemistryBiology01 natural sciencesDNA sequencing0104 chemical scienceslaw.invention03 medical and health scienceschemistry.chemical_compound0302 clinical medicinechemistrylawPolymorphism (computer science)GeneticsCapra hircus030216 legal & forensic medicineRestriction fragment length polymorphismGeneGenetics (clinical)Polymerase chain reactionDNABMC Genetics
researchProduct

Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle, sheep, goat, roe buck and deer by PCR-RFLP

2004

Abstract Background As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. Results Interspecies-specific DNA sequence polymorphisms in the mitochondrial cytochrome b gene were analyzed using PCR-RFLP technology to determine the source (i.e., species) of blood traces obtained from a leaf. Conclusions The method presented can be used for the discrimination of cattle (Bos taurus), sheep (Ovis aries), goat (Capra hircus), roe buck (Capreolus capreolus) and red deer (Ce…

Sex Determination AnalysisPolymorphism GeneticSheeplcsh:QH426-470Methodology ArticleDeerGoats590RuminantsCytochromes bDNA MitochondrialPolymerase Chain ReactionMitochondriaMitochondrial Proteinslcsh:GeneticsMolecular Diagnostic TechniquesSpecies SpecificityAnimalsCattlePolymorphism Restriction Fragment LengthBMC Genetics
researchProduct