Compensation of missing wedge effects with sequential statistical reconstruction in electron tomography.

2014

Electron tomography (ET) of biological samples is used to study the organization and the structure of the whole cell and subcellular complexes in great detail. However, projections cannot be acquired over full tilt angle range with biological samples in electron microscopy. ET image reconstruction can be considered an ill-posed problem because of this missing information. This results in artifacts, seen as the loss of three-dimensional (3D) resolution in the reconstructed images. The goal of this study was to achieve isotropic resolution with a statistical reconstruction method, sequential maximum a posteriori expectation maximization (sMAP-EM), using no prior morphological knowledge about …

Tavalliset taapertajatkin ovat kilpailemisen kannattajia : kilpailun mielekkyys ei ole sidoksissa liikuntataitoon

2000