0000000001140494

AUTHOR

Kaomei Guan

0000-0002-0753-3083

showing 1 related works from this author

Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles.

2008

Spermatogonial stem cells (SSCs) isolated from the adult mouse testis and cultured have been shown to respond to culture conditions and become pluripotent, so called multipotent adult germline stem cells (maGSCs). microRNAs (miRNAs) belonging to the 290 and 302 miRNA clusters have been previously classified as embryonic stem cell (ESC) specific. Here, we show that these miRNAs generally characterize pluripotent cells. They are expressed not only in ESCs but also in maGSCs as well as in the F9 embryonic carcinoma cell (ECC) line. In addition, we tested the time-dependent influence of different factors that promote loss of pluripotency on levels of these miRNAs in all three pluripotent cell t…

KOSRPluripotent Stem CellsEmbryologyCellular differentiationMice Inbred StrainsMice TransgenicEmbryoid bodyBiologyCell Line03 medical and health sciencesMice0302 clinical medicineCell Line TumorGeneticsAnimalsRNA MessengerInduced pluripotent stem cellMolecular BiologyCell potencyEmbryonic Stem Cells030304 developmental biologyGenetics0303 health sciencesReverse Transcriptase Polymerase Chain ReactionGene Expression ProfilingStem CellsAge FactorsObstetrics and GynecologyCell BiologyEmbryonic stem cellCell biologyMice Inbred C57BLAdult Stem CellsMicroRNAsGerm CellsReproductive Medicine030220 oncology & carcinogenesisStem cellOctamer Transcription Factor-3Developmental BiologyAdult stem cellMolecular human reproduction
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