0000000001153673

AUTHOR

Maria Moriondo

showing 2 related works from this author

Culture and Real-time Polymerase Chain reaction sensitivity in the diagnosis of invasive meningococcal disease: Does culture miss less severe cases?

2019

BackgroundInvasive meningococcal disease (IMD) is a highly lethal disease. Diagnosis is commonly performed by culture or Realtime-PCR (qPCR).AimsOur aim was to evaluate, retrospectively, whether culture positivity correlates with higher bacterial load and fatal outcome. Our secondary aim was to compare culture and qPCR sensitivity.MethodsThe National Register for Molecular Surveillance was used as data source. Cycle threshold (CT), known to be inversely correlated with bacterial load, was used to compare bacterial load in different samples.ResultsThree-hundred-thirteen patients were found positive for Neisseria meningitidis by qPCR, or culture, or both; 41 died (case fatality rate 13.1%); 1…

Bacterial DiseasesMale0301 basic medicinePhysiologyAntibioticsCell Culture TechniquesMeningococcal DiseaseNeisseria meningitidisPathology and Laboratory Medicinemedicine.disease_causeNervous SystemSeverity of Illness IndexGastroenterology0302 clinical medicineCerebrospinal fluidAntibioticsInfectious Diseases of the Nervous SystemBiochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)Case fatality rateMedicine and Health Sciences030212 general & internal medicineChildFalse Negative ReactionsCerebrospinal FluidMultidisciplinaryAntimicrobialsNeisseria meningitidisQRDrugsBody FluidsBacterial PathogensBloodInfectious DiseasesReal-time polymerase chain reactionNeurologyMedical MicrobiologyChild PreschoolMedicineFemaleAnatomyPathogensNeisseriaMeningitisResearch ArticleDNA Bacterialmedicine.medical_specialtyAdolescentmedicine.drug_classScienceInflammatory Diseases030106 microbiologyMeningitis MeningococcalReal-Time Polymerase Chain ReactionMeningococcal diseaseMicrobiologySensitivity and SpecificitySepsisYoung Adult03 medical and health sciencesSigns and SymptomsDiagnostic MedicineMicrobial ControlSepsisInternal medicinemedicineHumansMeningitisMicrobial PathogensRetrospective StudiesPharmacologyBacteriabusiness.industryOrganismsInfant NewbornBiology and Life SciencesInfantmedicine.diseaseBacterial Loadbusiness
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VP7 and VP4 Sequence Analyses of Rotavirus Strains From Italian Children With Viraemia and Acute Diarrhoea

2010

Background: Rotavirus has a high genetic variability. Point mutations, accumulating at a high rate, and genetic reassortment events have been well-documented. Viremia occurs commonly in children with acute rotavirus diarrhoea. However, information on genetic characterization of strains associated with systemic infection is poor. Objective: We evaluated prospectively children hospitalized for acute rotavirus diarrhoea and genotyped strains obtained from blood and stool samples. Nucleotide sequences within the VP4 ad VP7 genes of strains obtained from blood and stool specimens of the same patient were compared. Methods: Study subjects were 11 children admitted with acute rotavirus diarrhoea, …

DiarrheaRotavirusSettore MED/07 - Microbiologia E Microbiologia Clinicasequence analysisSettore MED/17 - Malattie InfettiveGenotypeSequence analysisvirusesReoviridaeBiologymedicine.disease_causeVirusNeutralizationRotavirus InfectionsFecesSettore MED/38 - Pediatria Generale E Specialisticafluids and secretionschildrenRotavirusGenotypemedicineHumansAmino Acid SequenceViremiaAmino AcidsAntigens ViralrotaviruGastroenterologyGenetic VariationInfantbiology.organism_classificationVirologyVP7DiarrheaAmino Acid SubstitutionItalyVP4Child PreschoolPediatrics Perinatology and Child HealthCapsid ProteinsViral diseasemedicine.symptom
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