0000000001162406

AUTHOR

Chrystala Constantinidou

showing 2 related works from this author

Comparison of CRISPR and marker based methods for the engineering of phage T7

2020

With the recent rise in interest in using lytic bacteriophages as therapeutic agents, there is an urgent requirement to understand their fundamental biology to enable the engineering of their genomes. Current methods of phage engineering rely on homologous recombination, followed by a system of selection to identify recombinant phages. For bacteriophage T7, the host genescmkortrxhave been used as a selection mechanism along with both type I and II CRISPR systems to select against wild-type phage and enrich for the desired mutant. Here we systematically compare all three systems; we show that the use of marker-based selection is the most efficient method and we use this to generate multiple …

0303 health sciences030306 microbiologyMutantComputational biologyBiologybiology.organism_classificationGenomeBacteriophage03 medical and health sciencesLytic cycleCRISPRHomologous recombinationGeneSelection (genetic algorithm)030304 developmental biology
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Comparison of CRISPR and Marker-Based Methods for the Engineering of Phage T7

2020

This article belongs to the Section Bacterial Viruses.

0301 basic medicineGenetic Markersviruses030106 microbiologyMutantlcsh:QR1-502t7Computational biologyGenome ViralBiologyGenomeArticlelcsh:MicrobiologyBacteriophage03 medical and health sciencesbacteriophageVirologyBacteriophage T7CRISPRClustered Regularly Interspaced Short Palindromic RepeatsGenomescrisprBacteriophageGeneSelection (genetic algorithm)Gene EditingQHT7Viral Tail Proteinsbiology.organism_classificationBacteriòfags3. Good healthQRtail fibres030104 developmental biologyInfectious DiseasesLytic cycleCRISPRMutationTail fibresCRISPR-Cas SystemsHomologous recombinationGenèticaViruses
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