DNA TYPING OF BLOODSTAIN: A CASEWORK OF MORTAL CRASH

Since 1997 the analysis of DNA recovered from handled objects, or trace DNA has become routine and is frequently demanded from crime scene examination. However, this analysis often produces unpredictable results. The factor affecting the recovery of full profiles are numerous, and as demonstrated in casework and research, retrieving useable profiles from trace DNA exhibits is not guaranteed. There are numerous factors that affect the rate of recovery of full profiles, such as the ability of the individual to shed cells, the substrate surface, the time of contact and delay until analysis, and the environment. In addition to these external conditions, there are many varying methods used to co…

DNA typing from vaginal smears slides and stained sperm-positive evidence in suspected rape cases.

Rape is a largely un-witnessed crime, and may be difficult to obtained statements from the victim, since most of the victims suffer from emotional damage as well as physical injury. The physical examination by forensic specialist is extremely important.

Mitochondrial DNA deletion and male infertility

Abstract The term “male infertility” does not constitute a defined clinical syndrome, but rather, a collection of different conditions exhibiting a variety of aetiologies and varying diagnosis. In men, oligozoospermia, asthenozoospermia, teratozoospermia and azoospermia are the main causes of infertility. The present research is aimed to investigate if mtDNA deletions can cause sperm defects in idiopathic astenozoospermic patients with different sperm motility and sperm concentration. The incidence of deletions in mtDNA (mtDNA) was co-related with diagnostic categorization of male infertility when 6.6kb out of the 16.6kb of the mitochondrial genome was amplified from whole sperm samples, f…

Study of the variability of an endophytic Acremonium population in symptomless grapevines.

IDENTIFICAZIONE E UTILIZZAZIONE DI MARCATORI MOLECOLARI NELLA SISTEMATICA DEI FITOSEIDI (PARASITIFORMES, PHYTOSEIIDAE)

Astenozoospermy and mitochondrial DNA

Male infertility, inability to fecundate the oocyte, is due to alterations of sperm characteristics (low number, low motility, altered morphology), to the quantity of semen or to the presence of alterations in the male genital line. 75% of infertile men deal with untreatable sub-fertility. Semen of 200 idiopathic astenozoospermic patients and individuals with normal seminal parameters as control, were analyzed. Results showed that mtDNA and Y chromosome regions from the motile fractions of patients and controls gave amplifications, while, the non-motile fractions of the same seminal samples did not give amplifications of ND5, ND6 and regions inside the D-loop (Carra E. et al.2002, 2004). We…

DNA Typing of Blood Stain: a casework of mortal crash

ASTENOZOOSPERMY AND MITOCHONDRIAL DNA

A METHOD FOR STUDYING GENETICS OF THE PREDATORY MITE PHYTOSEIULUS PERSIMILIS

Two DNA extraction and purification procedures for single mite analysis, organic (phenol-chloroform) method and the Chelex® -100 DNA extraction procedure, have been compared concerning extraction efficiency, DNA purity and DNA suitability for amplification. Phenol-chloroform extraction takes a relatively long period of time (5 days) and involves the use of toxic and hazardous chemical reagents but it produces consistent results. Chelex is a chelating resin that has a high affinity for polyvalent metal ions, Singer-Sam et al. postulated that boiling a sample in the presence of chelex prevents the degradation of DNA. Both method were evaluated to extract DNA from single frozen mite or individ…

DNA extraction from phytoseiid specimens preserved on glass slide

Mites belonging to the family of Phytoseiidae are important biocontrol agents of phytophagous mites (McMurtry and Croft, 1997). But the species used for controlling harmful mites have to be correctly identified. Sometimes using the traditional identification methods it is difficult or uncertain to identify a species especially as various Authors use different characters, very often not stable in the time. In the 60’s M.me Athias-Henriot (Athias-Henriot 1968; 1969) took into consideration the insemination apparatus that is considered a stable character in the time. According to her, the shape of this apparatus could be considered a major criterion for genus distinction; but other scientists …