0000000001194378
AUTHOR
Sascha Theobald
Manganese-dependent growth of Oenococci
Abstract Oenococci are usually grown in complex media supplemented with tomato juice. The manganese concentration of tomato juice is about 10 µM, which shows a growth-stimulating effect. Unexpectedly, we found that high concentrations of manganese (34 mM) could replace tomato juice. At this concentration, far exceeding the concentration in growth media, several Oenococcus oeni strains yielded the same cell density as in the presence of tomato juice. The observed significant differences in the manganese dependence should also have an impact on the growth of oenococci in must and wine, where they are used as starter cultures for the removal of malic acid.
Influence of epigallocatechin gallate and phenolic compounds from green tea on the growth of Oenococcus oeni
Aims: To investigate the effect of phenolic compounds on the growth of Oenococcus oeni. Methods and Results: Oenococci are usually grown in media often supplemented with complex additives such as tomato juice. In order to improve our knowledge about the growth requirements of oenococci, we added several juices and leaf extracts such as green tea to the culture media and screened them for growth-stimulating substances to substitute complex supplements such as juices by more defined components. We found that also green tea could cause a growth stimulation of Oenococcus oeni strain B2. Conclusions: Further experiments showed that the stimulating effect was as a result of the phenolic compou…