Author: Annemie Boets

0000000001211172

AUTHOR

Annemie Boets

showing 1 related works from this author

Screening and identification ofvipgenes inBacillus thuringiensisstrains

2009

Aims: To identify known vip genes and to detect potentially novel vip genes in a collection of 507 strains of Bacillus thuringiensis. Methods and Results: Following a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) strategy, four restriction patterns were found within the vip1 family: vip1Aa1, vip1Ba1/vip1Ba2 and vip1Ca. In the screening of vip2 genes, patterns similar to those of vip2Aa1, vip2Ba1/vip2Ba2 and vip2Ac1 genes were observed. Patterns for vip3Aa1, vip3Ae2 and vip3Af1 were found among vip3 genes. Two new patterns revealed novel vip1 and vip3A genes. The observed frequency of genes belonging to vip1 and vip2 families was around 10%, whereas 48·9% of…

GeneticsBacillaceaeVasoactive intestinal peptideBacillus thuringiensisSequence Analysis DNAGeneral MedicineBiologybiology.organism_classificationPolymerase Chain ReactionApplied Microbiology and BiotechnologyGenetic analysisMolecular biologylaw.inventionBacterial ProteinsGene FrequencylawBacillus thuringiensisGene expressionRestriction fragment length polymorphismGenehormones hormone substitutes and hormone antagonistsPolymerase chain reactionDNA PrimersBiotechnologyJournal of Applied Microbiology

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PEPhysical Sciences and Engineering
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