0000000001248087

AUTHOR

Morgan Guilbaud

showing 4 related works from this author

Evidence of autoinduction heterogeneity via expression of the agr system of listeria monocytogenes at the single-cell level

2011

ABSTRACT To investigate if the primary function of the Agr system of Listeria monocytogenes is to monitor cell density, we followed Agr expression in batch cultures, in which the autoinducer concentration was uniform, and in biofilms. Expression was heterogeneous, suggesting that the primary function of Agr is not to monitor population density.

listeria monocytogenes growth and developmentbiofilms growth and developmentBiologyCellular levelmedicine.disease_causeApplied Microbiology and Biotechnology[ SDV.EE ] Life Sciences [q-bio]/Ecology environmentMicrobiologygene expression regulation bacterial03 medical and health sciencesListeria monocytogenesBacterial ProteinsCell densitymedicinelisteria monocytogenes genetics030304 developmental biology[SDV.EE]Life Sciences [q-bio]/Ecology environment0303 health sciencesEcology030306 microbiologyBiofilmquorum sensingGene Expression Regulation Bacterialbiochemical phenomena metabolism and nutritionbacterial infections and mycosesListeria monocytogenesbacterial proteins biosynthesisBiofilmsFood MicrobiologybacteriaAutoinducerFunction (biology)Food ScienceBiotechnology
researchProduct

Exploring the diversity of listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of …

2015

ABSTRACT Listeria monocytogenes is involved in food-borne illness with a high mortality rate. The persistence of the pathogen along the food chain can be associated with its ability to form biofilms on inert surfaces. While most of the phenotypes associated with biofilms are related to their spatial organization, most published data comparing biofilm formation by L. monocytogenes isolates are based on the quantitative crystal violet assay, which does not give access to structural information. Using a high-throughput confocal-imaging approach, the aim of this work was to decipher the structural diversity of biofilms formed by 96 L. monocytogenes strains isolated from various environments. Pr…

[SDV.BIO]Life Sciences [q-bio]/Biotechnology[SDV]Life Sciences [q-bio]chaîne alimentairestrain originmicroscopie confocale à balayage lasermedicine.disease_causeApplied Microbiology and Biotechnologybiofilmchemistry.chemical_compound[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyPathogenmorphotypeComputingMilieux_MISCELLANEOUS0303 health sciencesGrowth mediumMicroscopy ConfocalEcologyMicrobiology and ParasitologydescripteurMicrobiologie et Parasitologieenvironnementphénotype[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitologymotilityanalyse quantitativeoptimizationBiotechnologyagent pathogènePseudomonas-aeruginosa biofilm;pathogen;lineage;growth-condition;extracellular DNA;strain origin;quantification;motility;hydrophobicity;optimizationBiotechnologiesBiologyHoneycomb likeMicrobiology03 medical and health sciencesListeria monocytogenesgrowth-conditionConfocal laser scanning microscopymedicineCrystal violetPseudomonas-aeruginosa biofilm030304 developmental biologydiversitéhydrophobicity030306 microbiologyBiofilm[ SDV.BIO ] Life Sciences [q-bio]/Biotechnologybiochemical phenomena metabolism and nutritionExtracellular dnaListeria monocytogenesquantificationHigh-Throughput Screening AssayschemistryBiofilmsFood MicrobiologyMicrobial Interactionslisteria monocytogènesFood Sciencepathogenlineageextracellular DNA
researchProduct

Single cell analysis evidence Heterogeneous expression of the Agr communication system of Listeria monocytogenes

2012

http://prodinra.inra.fr/record/271694EAMERSCT3 EJ3; Communication, often referred as Quorum Sensing, is involved in the adaptation of most bacteria to their environment. In the genus Listeria, the agr communication system affects the biology of Listeria monocytogenes during saprophytic life (biofilm formation) and during infection. In the present study, we investigated in situ agr expression of six isolates of L. monocytogenes by combining gfp reporters, flow cytometry and fluorescent microscopy during plantonic growth and dynamic flow cell biofilm set ups. Unexpectedly, during growth in TSB homogenised liquid cultures incubated at 25°C, while auto-inducer concentration was uniform, statist…

[SDV] Life Sciences [q-bio][ SDV ] Life Sciences [q-bio]listeria monocytogenes[SDV]Life Sciences [q-bio]bacteriabiochemical phenomena metabolism and nutritionheterogeneitysingle cell
researchProduct

Biofilms structural diversity of 100 strains of the food-borne pathogen Listeria monocytogenes

2012

International audience

[SDV] Life Sciences [q-bio][ SDV ] Life Sciences [q-bio][SDV]Life Sciences [q-bio]ComputingMilieux_MISCELLANEOUS
researchProduct