0000000001253470

AUTHOR

Pablo Llop

showing 6 related works from this author

Erwinia piriflorinigrans sp. nov., a novel pathogen that causes necrosis of pear blossoms

2010

Eight Erwinia strains, isolated from necrotic pear blossoms in València, Spain, were compared with reference strains of Erwinia amylovora and Erwinia pyrifoliae, both of which are pathogenic to species of pear tree, and to other species of the family Enterobacteriaceae using a polyphasic approach. Phenotypic analyses clustered the novel isolates into one phenon, distinct from other species of the genus Erwinia, showing that the novel isolates constituted a homogeneous phenotypic group. Rep-PCR profiles, PCR products obtained with different pairs of primers and plasmid contents determined by restriction analysis showed differences between the novel strains and reference strains of E. amylovo…

AD-HOC-COMMITTEEBACTERIALFIRE BLIGHT PATHOGENErwiniaPolymerase Chain ReactionErwinia pyrifoliaePyrusRNA Ribosomal 16SCluster Analysis[SDV.BDD]Life Sciences [q-bio]/Development BiologyPhylogenyNESTED-PCRBase Composition0303 health sciencesPEARbiologyPhylogenetic treeNucleic Acid Hybridizationfood and beveragesGeneral MedicineEnterobacteriaceaeBacterial Typing TechniquesTHERMAL-DENATURATIONPlasmidsDNA BacterialGenotypeMolecular Sequence DataDNA RibosomalMicrobiologyMicrobiology03 medical and health sciencesBacterial ProteinsPhylogeneticsDEOXYRIBONUCLEIC-ACIDEcology Evolution Behavior and SystematicsPlant Diseases030304 developmental biologyIDENTIFICATIONSEQUENCES030306 microbiologyAMYLOVORASequence Analysis DNADNARibosomal RNAbiochemical phenomena metabolism and nutrition16S ribosomal RNAbiology.organism_classificationMolecular TypingSpainErwiniabacteria
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Genetic islands in pome fruit pathogenic and non-pathogenic Erwinia species and related plasmids

2015

New pathogenic bacteria species belonging to the genus Erwinia associated with pome fruit trees (Erwinia pyrifoliae, E. piriflorinigrans, E. uzenensis) have been increasingly described in the last years, and comparative analyses have found that all these species share several genetic characteristics. Studies at different level (whole genome comparison, virulence genes, plasmid content, etc.) show a high intraspecies homogeneity (i.e. among E. amylovora strains) and also abundant similarities appear between the different Erwinia species: presence of plasmids of similar size in the pathogenic species; high similarity in several genes associated with exopolysaccharide production and hence, wit…

Microbiology (medical)Genetic diversity as resourceMini Reviewlcsh:QR1-502Gene interactionErwiniaMicrobiologyGenometransfer elementslcsh:MicrobiologyErwinia pyrifoliaePlasmidGene interactionH20 Plant diseasesEcological nicheGeneticsGenetic diversityErwinia genusbiologygenetic diversityPome fruitsbiology.organism_classificationTransfer elementsHorizontal gene transferErwiniaGene similarityFrontiers in Microbiology
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Draft Genome Sequences of Dickeya sp. Isolates B16 (NIB Z 2098) and S1 (NIB Z 2099) Causing Soft Rot of Phalaenopsis Orchids

2015

ABSTRACT The genus Dickeya contains bacteria causing soft rot of economically important crops and ornamental plants. Here, we report the draft genome sequences of two Dickeya sp. isolates from rotted leaves of Phalaenopsis orchids.

PhalaenopsisbiologyGenus Dickeyafungitechnology industry and agriculturefood and beveragesmacromolecular substancesGene sequencebiology.organism_classificationBioinformaticsSoft rotcomplex mixturesGenomeU30 Research methodsBotanyOrnamental plantGeneticsDickeya sp.ProkaryotesPhalaenopsisH20 Plant diseasesDickeyaMolecular BiologyF30 Plant genetics and breedingGenome Announcements
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Rifampicin treatment of Blattella germanica evidences a fecal transmission route of their gut microbiota

2018

Eukaryotes have established symbiotic relationship with microorganisms, which enables them to accomplish functions that they cannot perform alone. In the German cockroach, Blattella germanica, the obligate endosymbiont Blattabacterium coexists with a rich gut microbiota. The transmission of Blattabacterium is vertical, but little is known about how the gut microbiota colonizes newborn individuals. In this study, we treated B. germanica populations with rifampicin, a broad-spectrum antibiotic, during two generations and analyzed gut bacterial composition and the Blattabacterium load in control and rifampicin-treated populations. Rifampicin exerted a drastic effect on gut microbiota compositi…

0301 basic medicineAdultMalemedicine.drug_class030106 microbiologyAntibioticsPopulationL73 Animal diseasesGut microbiotaGut floraL70 Veterinary science and hygieneL01 Animal husbandryApplied Microbiology and BiotechnologyMicrobiologydigestive systemMicrobiology03 medical and health sciencesBlattabacteriumFecesSymbiosismedicineAnimalsHumanseducationSymbiosisFecesPhylogenyGerman cockroacheducation.field_of_studyEndosymbiontEcologybiologyObligateBlattellidaebiology.organism_classificationAntibiotic treatmentAnti-Bacterial AgentsBacterial Typing TechniquesGastrointestinal MicrobiomeInsect symbiosisRifampinFlavobacteriaceaeMicrobiota transmission
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In silico evaluation of molecular probes for detection and identification of Ralstonia solanacearum and Clavibacter michiganensis subsp. sepedonicus.

2004

Ralstonia solanacerum and Clavibacter michiganensis subsp. sepedonicus are the two most relevant bacterial pathogens of potato for which a large number of molecular diagnostic methods using specific DNA sequences have been developed. About one hundred oligonucleotides have been described and thoroughly tested experimentally. After having compiled and evaluated all these primers and probes in silico to check their specificity, many discrepancies were found. A detailed analysis permitted the recognition of different possible reasons for such discrepancies: sequencing errors in public sequences, wrong supposed specificity (sometimes due to more recent sequences than the oligonucleoticles being…

Ralstonia solanacearumbiologyBase SequenceOligonucleotideIn silicofood and beveragesComputational biologybiology.organism_classificationApplied Microbiology and BiotechnologyMicrobiologySensitivity and SpecificityDNA sequencingMicrobiologyRalstoniaActinomycetalesRalstonia solanacearumTypingMolecular probeOligonucleotide ProbesClavibacter michiganensisEcology Evolution Behavior and SystematicsDNA PrimersSolanum tuberosumSystematic and applied microbiology
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Caracterización molecular de la pérdida del poder patógeno en Agrobacterium tumefaciens

2003

Agrobacterium tumefaciens es una bacteria fitopatógena causante de tumores en el cuello y raíces de muchas especies vegetales de interés económico. En los viveros e invernaderos es donde más daños produce, al aparecer episodios epidémicos que pueden impedir la comercialización de producciones enteras, porque las plantas afectadas por esta enfermedad no pueden ser comercializadas. Los estudios epidemiológicos y de dinámica de poblaciones del patógeno son poco abundantes y han incidido en aspectos del desarrollo y mantenimiento de la enfermedad producida por la bacteria. Aunque se ha señalado el predominio de cepas no patógenas frente a patógenas en ambientes en los que las cepas productoras …

TaxonomíanonePatogenicidadInstituto Valenciano de Investigaciones Agrarias58Características genotípicasTumores de las plantasPlantas huéspedesBacterias fitopatógenasAgrobacterium tumefaciensU30 Research methodsCaracterísticas fenotípicasCepas no patógenasRAPDsH20 Plant diseases
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