0000000001264068
AUTHOR
Tanja Andrejeff
showing 2 related works from this author
Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis
2014
Triangular shaped particles are an interesting research topic since there are three plasmonically active tips, which can be utilized in new molecular sensing application systems. In this research we used dielectrophoretic force to trap thiol-modified (5’- end) and Cy3-dye-labeled (3’-end) single-stranded DNA (ssDNA) oligonucleotides, size 40 nt (about 22 nm long), to the corners of the gold triangles. These gold triangles were 20 nm thick and side length was 1 μm. The trapping experiment was done under AC-circuit and the gathering of the oligos to the triangles was studied in situ under confocal microscope. The theoretical values for dielectrophoretic force with different voltages were simu…
Formalin treatment increases the stability and immunogenicity of coxsackievirus B1 VLP vaccine
2019
Type B Coxsackieviruses (CVBs) are a common cause of acute and chronic myocarditis, dilated cardiomyopathy and aseptic meningitis. However, no CVB-vaccines are available for human use. We have previously produced virus-like particles (VLPs) for CVB3 with a baculovirus-insect cell production system. Here we have explored the potential of a VLP-based vaccine targeting CVB1 and describe the production of CVB1-VLPs with a scalable VLP purification method. The developed purification method consisting of tangential flow filtration and ion exchange chromatography is compatible with industrial scale production. CVB1-VLP vaccine was treated with UV-C or formalin to study whether stability and immuno…