0000000001264456
AUTHOR
Ferran Garcia-pichel
showing 14 related works from this author
A New Niche for Anoxygenic Phototrophs as Endoliths
2018
ABSTRACT Anoxygenic phototrophic bacteria (APBs) occur in a wide range of aquatic habitats, from hot springs to freshwater lakes and intertidal microbial mats. Here, we report the discovery of a novel niche for APBs: endoliths within marine littoral carbonates. In a study of 40 locations around Isla de Mona, Puerto Rico, and Menorca, Spain, 16S rRNA high-throughput sequencing of endolithic community DNA revealed the presence of abundant phylotypes potentially belonging to well-known APB clades. An ad hoc phylogenetic classification of these sequences enabled us to refine the assignments more stringently. Even then, all locations contained such putative APBs, often reaching a significant pro…
Additional file 3: of Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and …
2019
Table S4. Taxonomic assignment and functional inferences based on phylogenetic placement for segregating OTUs for both bulk soils (cold and hot deserts). Rows colored in yellow correspond to OTUs for which inferred function was consistent with segregation from M. vaginatus. (DOCX 192 kb)
Additional file 2: of Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and …
2019
Table S1. Summary of SSU rRNA gene libraries analyzed from HSN and FB sample set and associated coverage and Îą-diversity indices. (DOCX 23 kb)
Additional file 2: of Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and …
2019
Table S1. Summary of SSU rRNA gene libraries analyzed from HSN and FB sample set and associated coverage and Îą-diversity indices. (DOCX 23 kb)
Possible nitrogen fertilization of the early Earth Ocean by microbial continental ecosystems
2018
While significant efforts have been invested in reconstructing the early evolution of the Earth’s atmosphere–ocean–biosphere biogeochemical nitrogen cycle, the potential role of an early continental contribution by a terrestrial, microbial phototrophic biosphere has been largely overlooked. By transposing to the Archean nitrogen fluxes of modern topsoil communities known as biological soil crusts (terrestrial analogs of microbial mats), whose ancestors might have existed as far back as 3.2 Ga ago, we show that they could have impacted the evolution of the nitrogen cycle early on. We calculate that the net output of inorganic nitrogen reaching the Precambrian hydrogeological system could hav…
In Situ X-Ray Tomography Imaging of Soil Water and Cyanobacteria From Biological Soil Crusts Undergoing Desiccation
2018
Biological soil crusts (biocrusts) are millimeter-sized microbial communities developing on the topsoils of arid lands that cover some 12% of Earth’s continental area. Biocrusts consist of an assemblage of mineral soil particles consolidated into a crust by microbial organic polymeric substances that are mainly produced by the filamentous bundle-forming cyanobacteria, among which Microcoleus vaginatus is perhaps the most widespread. This cyanobacterium is the primary producer for, and main architect of biocrusts in many arid soils, sustaining the development of a diverse microbial community. Biocrusts are only active when wet, and spend most of their time in a state of desiccated quiescence…
Diversity and mineral substrate preference in endolithic microbial communities from marine intertidal outcrops (Isla de Mona, Puerto Rico)
2017
14 pages; International audience; Endolithic microbial communities are prominent features of intertidal marine habitats, where they colonize a variety of substrates, contributing to their erosion. Almost 2 centuries worth of naturalistic studies focused on a few true-boring (euendolithic) phototrophs, but substrate preference has received little attention. The Isla de Mona (Puerto Rico) intertidal zone offers a unique setting to investigate substrate specificity of endolithic communities since various phosphate rock, limestone and dolostone outcrops occur there. High-throughput 16S rDNA genetic sampling, enhanced by targeted cultivation, revealed that, while euendolithic cyanobacteria were …
Additional file 1: of Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and …
2019
Table S2. Taxonomic assignments and functional inference based on phylogenetic placement for aggregating (cyanosphere) OTUs in the cold desert (HSN). Table S3. Taxonomic assignments and functional inference based on phylogenetic placement for aggregating (cyanosphere) OTUs in the hot desert (FB). (DOCX 38 kb)
Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and the formation of a nit…
2019
12 pages; International audience; Background Biological soil crusts (biocrusts) are a key component of arid land ecosystems, where they render critical services such as soil surface stabilization and nutrient fertilization. The bundle-forming, filamentous, non-nitrogen-fixing cyanobacterium Microcoleus vaginatus is a pioneer primary producer, often the dominant member of the biocrust microbiome, and the main source of leaked organic carbon. We hypothesized that, by analogy to the rhizosphere of plant roots, M. vaginatus may shape the microbial populations of heterotrophs around it, forming a specialized cyanosphere. Results By physically isolating bundles of M. vaginatus from biocrusts, we …
Additional file 4: of Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and …
2019
Table S5. Potential contaminants. Responding OTUs detected after amplification and sequencing of negative controls (nâ =â 5) without target Microcoleus bundles. (DOCX 17 kb)
Additional file 3: of Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and …
2019
Table S4. Taxonomic assignment and functional inferences based on phylogenetic placement for segregating OTUs for both bulk soils (cold and hot deserts). Rows colored in yellow correspond to OTUs for which inferred function was consistent with segregation from M. vaginatus. (DOCX 192 kb)
Large Blooms of Bacillales (Firmicutes) Underlie the Response to Wetting of Cyanobacterial Biocrusts at Various Stages of Maturity.
2018
ABSTRACT Biological soil crusts (biocrusts) account for a substantial portion of primary production in dryland ecosystems. They successionally mature to deliver a suite of ecosystem services, such as carbon sequestration, water retention and nutrient cycling, and climate regulation. Biocrust assemblages are extremely well adapted to survive desiccation and to rapidly take advantage of the periodic precipitation events typical of arid ecosystems. Here we focus on the wetting response of incipient cyanobacterial crusts as they mature from “light” to “dark.” We sampled a cyanobacterial biocrust chronosequence before (dry) and temporally following a controlled wetting event and used high-throug…
Additional file 1: of Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and …
2019
Table S2. Taxonomic assignments and functional inference based on phylogenetic placement for aggregating (cyanosphere) OTUs in the cold desert (HSN). Table S3. Taxonomic assignments and functional inference based on phylogenetic placement for aggregating (cyanosphere) OTUs in the hot desert (FB). (DOCX 38 kb)
Additional file 6: of Spatial segregation of the biological soil crust microbiome around its foundational cyanobacterium, Microcoleus vaginatus, and …
2019
Table S6. NMDS plot coordinates. The corresponding plot is displayed in Fig. 3a (DOCX 18 kb)