0000000001280324

AUTHOR

Luca Braga

showing 1 related works from this author

Transgene detection by digital droplet PCR

2014

Somatic gene therapy is a promising tool for the treatment of severe diseases. Because of its abuse potential for performance enhancement in sports, the World Anti-Doping Agency (WADA) included the term 'gene doping' in the official list of banned substances and methods in 2004. Several nested PCR or qPCR-based strategies have been proposed that aim at detecting long-term presence of transgene in blood, but these strategies are hampered by technical limitations. We developed a digital droplet PCR (ddPCR) protocol for Insulin-Like Growth Factor 1 (IGF1) detection and demonstrated its applicability monitoring 6 mice injected into skeletal muscle with AAV9-IGF1 elements and 2 controls over a 3…

Genetics and Molecular Biology (all)Gene Identification and AnalysisGene TransferBiochemistryPolymerase Chain Reaction796 Athletic and outdoor sports and gamesMiceMedicine and Health SciencesTransgenesInsulin-Like Growth Factor IIntramuscularMedicine (all)QRDependovirusDependoviruMedicineGenetic VectorResearch ArticleBiotechnologyHumanScienceGenetic VectorsReproducibility of ResultIn Vitro TechniquesInjections IntramuscularInjectionsBiomaterialsMolecular GeneticsTransgeneAnimals; Dependovirus; Erythropoietin; Genetic Vectors; Humans; In Vitro Techniques; Injections Intramuscular; Insulin-Like Growth Factor I; Mice; Polymerase Chain Reaction; Reproducibility of Results; Transgenes; Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)Genetic ElementsGeneticsAnimalsHumansSports and Exercise MedicineMolecular Biology TechniquesMolecular BiologyErythropoietinClinical GeneticsBiochemistry Genetics and Molecular Biology (all)796 SportAnimalIn Vitro TechniqueGene AmplificationBiology and Life SciencesReproducibility of ResultsHuman GeneticsDNAAgricultural and Biological Sciences (all)Mutation
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