A TEST FOR REPRODUCTIVE SEPARATION OF ALTERNATE GENERATIONS IN A BIENNIAL SPIDER, ARANEUS DIADEMATUS (ARANEAE, ARANEIDAE)

In Denmark, two seemingly distinct size-classes, 3rd and 4th instar juveniles and repro- ductive adults, of Araneus diadematus are found during every breeding season in autumn, indicating a non-overlapping biennial life-cycle. We tested the hypothesis that alternate generations might experience a degree of reproductive isolation, using the distribution of nuclear (allozyme) and maternal (mtDNA) genetic markers. Individuals of a locality behaved as belonging to a random mating population, irrespective of size. No differences were found between any size-class pairs, within and between 2 yr, or among geographically distant samples. Processes that may lead to this result are discussed: the bien…