0000000001300771
AUTHOR
Lara Dutra
showing 4 related works from this author
DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen
2020
Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a si…
Additional file 1: Table S1. of Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica
2018
Identification and locations of viruses isolated. (DOCX 34 kb)
Supplementary figure: DNA recovery from Droplet Digital PCR emulsions using Liquid Nitrogen
2020
Supplementary figure 1 – Applicability of LN2 method to break ddPCR emulsion. ddPCR reactions were prepared with ddPCR™ Supermix for Probes (no dUTP) and Droplet Generation Oil for Probes (three tubes on the left) or ddPCR™ Supermix for EvaGreen® and Droplet Generation Oil for EvaGreen® (three tubes on the right). The tubes (A) and (D) show the emulsion before using LN2 method, and tubes (B), (C), (E) and (F) shows the oil and water layers after breaking the emulsion.
Supplementary table: DNA recovery from Droplet Digital PCR emulsions using Liquid Nitrogen
2020
Supplementary table - Raw data from experiments studying DNA recovery from droplets using chemical and physical procedures.