0000000001301180
AUTHOR
ŁUkasz Michalczyk
Additional file 1 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 1: SM.01. Saturation plots for COI and ITS markers.
First insights into female sperm storage duration in tardigrades.
Female sperm storage is ubiquitous in the animal kingdom and it has been shown to be linked to several evolutionary processes, from postcopulatory sexual selection to dispersal. Here we report, for the first time, long-term sperm storage in females of the tardigrade Macrobiotus polonicus. Females, isolated after a short contact with a male, were able to use the stored sperm for up to 5 weeks (mean of 2 weeks), which translates to a considerable proportion of female post-mating longevity under controlled laboratory conditions (60% on average). Our study provides the first insights into the duration of sperm storage, an underexplored feature of the reproductive biology of tardigrades. Additio…
New multilocus phylogeny reorganises the family Macrobiotidae (Eutardigrada) and unveils complex morphological evolution of the Macrobiotus hufelandi group
The family Macrobiotidae is one of the most speciose and diverse groups among tardigrades. Although there have been attempts to reconstruct the phylogeny of this family, the evolutionary relationships within Macrobiotidae are only superficially determined as available genetic data cover only a small fraction of this vast group. Here, we present the first extensive molecular phylogeny of the family based on four molecular markers (18S rRNA, 28Sr RNA, ITS-2 and COI) associated with detailed morphological data for the majority of taxa. The phylogenetic analysis includes nearly two hundred sequences representing more than sixty species, including sixteen taxa that have never been sequenced and/…
Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
AbstractThe taxonomy of many groups of meiofauna is challenging due to their low number of diagnostic morphological characters and their small body size. Therefore, with the advent of molecular techniques that provide a new source of traits, many cryptic species have started to be discovered. Tardigrades are not an exception, and many once thought to be cosmopolitan taxa are being found to be complexes of phenotypically similar species. Macrobiotus pallarii Maucci, 1954 was originally described in South Italy and has been subsequently recorded in Europe, America, and Asia. This allegedly wide geographic range suggests that multiple species may be hidden under this name. Moreover, recently, …
Resolving the systematics of Richtersiidae by multilocus phylogeny and an integrative redescription of the nominal species for the genus Crenubiotus (Tardigrada)
AbstractThe family Richtersiidae, although established recently with the use of phylogenetic methods, was considered potentially paraphyletic at the time of its erection. Until now, the family comprised four genera, Richtersius, Diaforobiotus, Adorybiotus and a newly erected genus Crenubiotus. However, the genetic characterisation for the latter two genera was very limited or absent. To address concerns about the phylogenetic affinity of these two genera, we present a multilocus phylogeny of the families Richtersiidae and Murrayidae based on four molecular markers (18S rRNA, 28S rRNA, ITS-2 and COI). Our results show a distinct evolutionary lineage composed of Adorybiotus and Crenubiotus, w…
Additional file 11 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 11: SM.10. Results of PCA randomization tests.
Additional file 9 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 9: SM.08. Raw morphometric data for Macrobiotus ripperi sp. nov. from Finland (FI.066, additional population).
Additional file 4 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 4: SM.04a. R data for morphometric analysis.
Additional file 7 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 7: SM.06. Raw morphometric data for Macrobiotus pseudopallarii sp. nov. from Montenegro (ME.007, type population).
Additional file 8 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 8: SM.07. Raw morphometric data for Macrobiotus ripperi sp. nov. from Poland (PL.015, type population).
Additional file 9 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 9: SM.08. Raw morphometric data for Macrobiotus ripperi sp. nov. from Finland (FI.066, additional population).
Additional file 6 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 6: SM.05. Raw morphometric data for Macrobiotus pallarii Maucci, 1954 from the topotypic population (IT.337).
Additional file 7 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 7: SM.06. Raw morphometric data for Macrobiotus pseudopallarii sp. nov. from Montenegro (ME.007, type population).
Additional file 5 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 5: SM.04b. R script for morphometric analysis.
Additional file 6 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 6: SM.05. Raw morphometric data for Macrobiotus pallarii Maucci, 1954 from the topotypic population (IT.337).
Additional file 8 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 8: SM.07. Raw morphometric data for Macrobiotus ripperi sp. nov. from Poland (PL.015, type population).
Additional file 10 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 10: SM.09. Raw morphometric data for Macrobiotus margoae sp. nov. from USA (US.057, type population).
Additional file 3 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 3: SM.03. The p-distances between species of the Macrobiotus pallarii complex.
Additional file 2 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 2: SM.02. MrBayes analysis input file with the alignment.
Additional file 3 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 3: SM.03. The p-distances between species of the Macrobiotus pallarii complex.
Additional file 4 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 4: SM.04a. R data for morphometric analysis.
Additional file 5 of Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Additional file 5: SM.04b. R script for morphometric analysis.