0000000001304585

AUTHOR

Nathalie Cochennec-laureau

showing 15 related works from this author

Pmarg-pearlin is a matrix protein involved in nacre framework formation in the pearl oyster Pinctada margaritifera.

2011

11 pages; International audience; The shell of pearl oysters is organized in multiple layers of CaCO(3) crystallites packed together in an organic matrix. Relationships between the components of the organic matrix and mechanisms of nacre formation currently constitute the main focus of research into biomineralization. In this study, we characterized the pearlin protein from the oyster Pinctada margaritifera (Pmarg); this shares structural features with other members of a matrix protein family, N14/N16/pearlin. Pmarg pearlin exhibits calcium- and chitin-binding properties. Pmarg pearlin transcripts are distinctively localized in the mineralizing tissue responsible for nacre formation. More s…

OysterPteriidaeMolecular Sequence Dataengineering.materialBiologyMatrix (biology)010402 general chemistry01 natural sciencesBiochemistry03 medical and health sciencesProtein structureAnimal Shellsbiology.animalAnimalsAmino Acid SequencePinctadaRNA Messenger[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsMolecular Biologyglycoproteins030304 developmental biologyorganic matrix0303 health sciencesExtracellular Matrix ProteinsEcologyAragoniteOrganic ChemistryPinctada margaritiferabiology.organism_classificationbiomineralization[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterials0104 chemical sciencesCell biologyprotein structuresengineeringMolecular Medicinepearl oysterPearlBiomineralization
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Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2010

Abstract Background The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. Results We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Component…

0106 biological sciencesModels MolecularProteomicsProteome[SDV]Life Sciences [q-bio]Proteomics01 natural sciencesContig MappingMantle (mollusc)MargaritiferaIn Situ HybridizationGeneticsExpressed Sequence Tags0303 health sciencesMineralsbiologyPinctada margaritifera[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]ProteomeBiotechnologyResearch Articlelcsh:QH426-470Sequence analysislcsh:BiotechnologyMolecular Sequence Data010603 evolutionary biology03 medical and health sciencesCalcification Physiologiclcsh:TP248.13-248.65[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]GeneticsAnimals[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyAmino Acid SequencePinctadaRNA Messenger[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials030304 developmental biologyBase SequenceSequence Homology Amino AcidGene Expression ProfilingAnimal StructuresMolecular Sequence AnnotationSequence Analysis DNAbiology.organism_classification[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterialslcsh:GeneticsGene Expression RegulationEvolutionary biologyPinctadaBiomineralization
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Additional file 1 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file 1:Table S1: Summary of BlastX results of biomineralization-related protein in the EST P. margaritifera mantle database. A catalogue of 82 P. margaritifera mantle transcripts potentially implicated in the biomineralization process was constructed using BlastX (E-value < 10-3) with selected protein sequences identified from mollusks (bivalvia and gastropoda). (DOC 140 KB)

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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file 3 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 2

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Additional file 5 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 4

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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file 4 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 3

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Additional file 2 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 1

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Additional file 5 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 4

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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file 4 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 3

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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Additional file of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

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Additional file 2 of Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization

2021

Authors’ original file for figure 1

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